Clinical Laboratory, Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, Xi' an, China.
2Department of Tuberculosis, Beijing Key Laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beij.
New Microbiol. 2020 Oct;43(4):191-194.
Nontuberculous mycobacteria (NTM) are important environmental opportunistic pathogens of human and animals. Rapidly growing mycobacteria (RGM) are important emerging pathogens causing NTM infections. Unfortunately, the majority of microorganisms in the environment resist cultivation in the laboratory. The aim of the study was to investigate whether the nutrients in the medium have impact on the growth of RGM in vitro. We first assessed the growth rate of rapidly growing mycobacteria strains in broth medium with different dilutions, including M. abscessus, M. chelonae, and M. fortuitum. Our data demonstrate that the majority of M. abscessus, M. chelonae and M. fortuitum strains prefer to grow in nutrient-rich MH medium, whereas a small proportion of RGM strains grew faster in diluted culture medium. Our study identified that dilution culture has a different impact on recovery of various RGM strains.
非结核分枝杆菌（NTM）是人类和动物重要的环境机会致病菌。快速生长分枝杆菌（RGM）是导致 NTM 感染的重要新兴病原体。不幸的是，环境中的大多数微生物在实验室中难以培养。本研究旨在探讨培养基中的营养成分是否会影响 RGM 的体外生长。我们首先评估了不同稀释度肉汤培养基中快速生长分枝杆菌菌株的生长速度，包括脓肿分枝杆菌、龟分枝杆菌和偶发分枝杆菌。我们的数据表明，大多数脓肿分枝杆菌、龟分枝杆菌和偶发分枝杆菌菌株更喜欢在营养丰富的 MH 培养基中生长，而一小部分 RGM 菌株在稀释的培养基中生长更快。我们的研究表明，稀释培养对不同 RGM 菌株的回收有不同的影响。
