Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China.
J Gen Virol. 2021 Mar;102(3). doi: 10.1099/jgv.0.001538. Epub 2020 Dec 7.
Rabies is a lethal disease caused by Rabies lyssavirus, commonly known as rabies virus (RABV), and results in nearly 100 % death once clinical symptoms occur in human and animals. Long non-coding RNAs (lncRNAs) have been reported to be associated with viral infection. But the role of lncRNAs involved in RABV infection is still elusive. In this study, we performed global transcriptome analysis of both of lncRNA and mRNA expression profiles in wild-type (WT) and lab-attenuated RABV-infected mouse brains by using next-generation sequencing. The differentially expressed lncRNAs and mRNAs were analysed by using the edgeR package. We identified 1422 differentially expressed lncRNAs and 4475 differentially expressed mRNAs by comparing WT and lab-attenuated RABV-infected brains. Then we predicted the enriched biological pathways by the Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) database based on the differentially expressed lncRNAs and mRNAs. Our analysis revealed the relationships between lncRNAs and RABV-infection-associated immune response and ion transport-related pathways, which provide a fresh insight into the potential role of lncRNA in immune evasion and neuron injury induced by WT RABV.
狂犬病是一种致命疾病，由狂犬病弹状病毒引起，通常称为狂犬病病毒（RABV），一旦在人和动物中出现临床症状，几乎 100%导致死亡。长链非编码 RNA（lncRNA）已被报道与病毒感染有关。但是，lncRNA 在 RABV 感染中的作用仍不清楚。在这项研究中，我们通过下一代测序对野生型（WT）和实验室减毒 RABV 感染的小鼠大脑中的 lncRNA 和 mRNA 表达谱进行了全局转录组分析。使用 edgeR 包分析差异表达的 lncRNA 和 mRNA。通过比较 WT 和实验室减毒 RABV 感染的大脑，我们鉴定了 1422 个差异表达的 lncRNA 和 4475 个差异表达的 mRNA。然后，我们根据差异表达的 lncRNA 和 mRNA，利用基因本体论（GO）和京都基因与基因组百科全书（KEGG）数据库预测了富集的生物途径。我们的分析揭示了 lncRNA 与 RABV 感染相关的免疫反应和离子转运相关途径之间的关系，这为 lncRNA 在 WT RABV 诱导的免疫逃避和神经元损伤中的潜在作用提供了新的见解。
