Int J Prosthodont. 2020 Nov/Dec;33(6):620-628. doi: 10.11607/ijp.6643.
To evaluate the effectiveness of liquid disinfectant soaps for the reduction of microorganisms present on maxillary complete dentures.
The selected patients (N = 28) were randomly divided into four groups (n = 7), and each group underwent all four disinfection treatments in a different order. The disinfection treatments evaluated were: 0.5% sodium hypochlorite (positive control); Dettol liquid soap; Lifebuoy liquid soap; and phosphate-buffered saline solution (negative control). The patients were instructed to immerse their maxillary dentures in the disinfectant solution for 8 hours (overnight) for 7 days, with the solutions in a randomized sequence with a washout period of 1 week between solutions. Biofilm samples of the dental prostheses were obtained before and after each treatment with a sterile swab, and the microbiologic material was diluted and plated in selective media for Candida spp. Colony-forming unit count (CFU/mL) was performed in each group. One-way ANOVA with Welch correction was used for analysis, with Games-Howell post hoc test with a significance level of .05.
A 3-log reduction in microorganisms was considered effective compared to baseline. The highest incidence observed was for Candida albicans, which presented with a frequency between 66% and 92%, followed by C tropicalis, with a frequency between 7% and 33%, in all experimental groups. Sodium hypochlorite was able to reduce more than 3 log of microorganisms for all patients, showing high antifungal effectiveness for both C albicans and C tropicalis species. Regarding the experimental groups, both liquid soaps (Dettol and Lifebuoy) were effective in reducing the two types of microorganisms.
Liquid soaps were effective at reducing biofilm and may be an alternative for disinfection of removable partial dentures or complete dentures.
评估液体消毒剂肥皂对减少上颌全口义齿上存在的微生物的有效性。
选择的患者（N=28）被随机分为四组（n=7），每组以不同的顺序进行所有四种消毒处理。评估的消毒处理如下：0.5%次氯酸钠（阳性对照）；滴露液体肥皂；力士液体肥皂；和磷酸盐缓冲盐水溶液（阴性对照）。患者被指示将上颌义齿浸入消毒剂溶液中 8 小时（过夜），共 7 天，溶液随机排列，溶液之间有 1 周的洗脱期。在每次处理之前和之后，使用无菌拭子从牙修复体上获得生物膜样本，并用选择性培养基稀释并接种微生物材料以进行白色念珠菌属。组内进行 CFU/mL 集落形成单位计数。使用带有 Welch 校正的单因素方差分析进行分析，使用 Games-Howell 事后检验，显著性水平为.05。
与基线相比，微生物减少 3 个对数被认为是有效的。观察到的最高发生率是白色念珠菌，其频率在 66%至 92%之间，其次是热带念珠菌，其频率在 7%至 33%之间，所有实验组均如此。次氯酸钠能够减少超过 3 对数的微生物，对白色念珠菌和热带念珠菌两种物种均具有高度抗真菌作用。关于实验组，两种液体肥皂（滴露和力士）均能有效减少两种类型的微生物。
液体肥皂在减少生物膜方面是有效的，可能是可摘局部义齿或全口义齿消毒的替代方法。
