State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
Environ Res. 2021 Feb;193:110533. doi: 10.1016/j.envres.2020.110533. Epub 2020 Dec 5.
Combining nitrate/nitrite dependent anaerobic methane oxidation (n-DAMO) and anaerobic ammonium oxidation (Anammox) is a promising sustainable wastewater treatment technology, which simultaneously achieve nitrogen removal and methane emission mitigation. However, the practical application of n-DAMO has been greatly limited by its extremely slow growth-rate and low reaction rate. This work proposes an innovative Membrane BioTrickling Filter (MBTF), which consist of hollow fiber membrane for effective methane supplementation and polyurethane sponge as support media for the attachment and growth of biofilm coupling n-DAMO with Anammox. When steady state with a hydraulic retention time (HRT) of 6.00 h was reached, above 99.9% of nitrogen was removed from synthetic sidestream wastewater at a rate of 3.99 g N L d. This system presented robust capacity to withstand unstable partial nitritation effluent, achieving complete nitrogen removal at a varied nitrite to ammonium ratio in the range of 1.10-1.40. It is confirmed that n-DAMO and Anammox microorganisms jointly dominated the microbial community by pyrosequencing technology. The complete nitrogen removal potential at high-rate and efficient biomass retention (12.4 g VSS L) of MBTF offers promising alternative for sustainable wastewater treatment by the combination of n-DAMO and Anammox.
将硝酸盐/亚硝酸盐依赖型厌氧甲烷氧化（n-DAMO）和厌氧氨氧化（Anammox）结合是一种很有前途的可持续废水处理技术，可同时实现氮的去除和甲烷的排放缓解。然而，n-DAMO 的实际应用受到其极其缓慢的生长速率和低反应速率的极大限制。本研究提出了一种创新性的膜生物滴滤器（MBTF），该装置由中空纤维膜用于有效补充甲烷和聚氨酯海绵作为生物膜附着和生长的支撑介质，将 n-DAMO 与 Anammox 耦合。当达到水力停留时间（HRT）为 6.00 h 的稳定状态时，合成侧流废水中超过 99.9%的氮以 3.99 g N L d 的速率被去除。该系统具有强大的能力来承受不稳定的部分亚硝化流出物，在硝态氮与氨氮比值为 1.10-1.40 的范围内实现完全的氮去除。通过焦磷酸测序技术证实，n-DAMO 和 Anammox 微生物共同主导了微生物群落。MBTF 的高去除率和高效生物量保留（12.4 g VSS L）的完全氮去除潜力为 n-DAMO 和 Anammox 的结合提供了可持续废水处理的有前途的替代方案。
