Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
Departamento de Química, Universidade Estadual de Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, Paraná, Brazil.
Int J Biol Macromol. 2021 Jan 15;167:726-735. doi: 10.1016/j.ijbiomac.2020.12.012. Epub 2020 Dec 4.
Hydrogels based on pectin and cellulose nanocrystals (CNC) were used in our study to nucleation and growth of hydroxyapatite (HAp) by the biomimetic method. In this study, we evaluated the direct impact of the different percentages of CNC on pectin hydrogel and the influence of HAp obtained through two methods. CNC were obtained from HCl hydrolysis following chemical functionalization through vinyl groups. The percentage of CNC positively induces thermal stability, mechanical properties and HAp mineralization from biomimetic using simulated body fluid (1.5 SBF). Hydrogels with 5% of CNC showed a higher amount of HAp immersed for 14 days, about 28% of HAp. The obtained hydrogels were compared with hydrogels containing 20% of HAp nanoparticles obtained by chemical precipitation. Biocompatibility of the hydrogels was evaluated by cell viability using fibroblasts (L929). In general, the hydrogels obtained through the biomimetic method show slightly larger biocompatibility compared to the hybrid hydrogels obtained from chemical precipitation.
本研究使用基于果胶和纤维素纳米晶体（CNC）的水凝胶通过仿生方法实现羟基磷灰石（HAp）的成核和生长。在本研究中，我们评估了不同百分比 CNC 对果胶水凝胶的直接影响，以及通过两种方法获得的 HAp 的影响。CNC 通过化学官能化的乙烯基基团从 HCl 水解中获得。CNC 的百分比通过使用模拟体液（1.5 SBF）从仿生学正向诱导热稳定性、机械性能和 HAp 矿化。含有 5% CNC 的水凝胶在浸泡 14 天后显示出更高的 HAp 含量，约为 28%的 HAp。将获得的水凝胶与通过化学沉淀获得的含有 20% HAp 纳米颗粒的水凝胶进行比较。通过使用成纤维细胞（L929）评估细胞活力来评估水凝胶的生物相容性。一般来说，与通过化学沉淀获得的杂交水凝胶相比，通过仿生方法获得的水凝胶显示出稍高的生物相容性。
