O'Brien Stephen J, Netz Uri, Hallion Jacob, Bishop Campbell, Stephen Vincent, Burton James, Paas Mason, Feagins Kayla, Pan Jianmin, Rai Shesh N, Galandiuk Susan
Price Institute of Surgical Research, The Hiram C. Polk Jr. MD Department of Surgery, University of Louisville School of Medicine, Louisville, KY 40292, USA.
Price Institute of Surgical Research, The Hiram C. Polk Jr. MD Department of Surgery, University of Louisville School of Medicine, Louisville, KY 40292, USA; Department of Surgery, Soroka University Medical Center, Beer Sheva, Israel.
Transl Oncol. 2021 Jan;14(1):100962. doi: 10.1016/j.tranon.2020.100962. Epub 2020 Dec 4.
Current serological surveillance markers to monitor colorectal cancer (CRC) or colorectal advanced adenomas (CAA) are hampered by poor sensitivity and specificity. The aim of this study is to identify and validate a panel of plasma microRNAs which change in expression after resection of such lesions.
A prospectively maintained colorectal surgery database was queried for patients in whom both pre- and post-procedural serum samples had been obtained. An initial screening analysis of CRC and CAA patients (5 each) was conducted using screening cards for 380 miRNAs. Four identified miRNAs were combined with a previously described panel of 7 miRNAs that were diagnostically predictive of CRC and CAA. Differential miRNA expression was assessed using quantitative real-time polymerase chain reaction(qRT-PCR).
Fifty patients were included (n = 27 CRC, n = 23 CAA). There was no difference in age, gender, or race profile of CRC patients compared to CAA patients. Six miRNA were significantly increased after CRC resection (miR-324, let7b, miR-454, miR-374a, miR-122, miR-19b, all p<0.05), while three miRNAs were significantly increased following CAA resection (miR-454, miR-374a, miR-122, all p<0.05). Three miRNA were increased in common for both (miR-454, miR-374a, miR-122).
The expression of miRNAs associated with neoplasia (either CRC or CAA) was significantly increased following surgical resection or endoscopic removal of CRC or CAA. Future studies should focus on the evaluation of these miRNAs in CRC and CAA prognosis.
目前用于监测结直肠癌（CRC）或结直肠高级别腺瘤（CAA）的血清学监测标志物存在敏感性和特异性较差的问题。本研究的目的是鉴定并验证一组在切除此类病变后表达发生变化的血浆微小RNA。
查询前瞻性维护的结直肠手术数据库，以获取术前和术后均采集了血清样本的患者。使用针对380种微小RNA的筛选卡，对CRC和CAA患者（各5例）进行了初步筛选分析。将鉴定出的4种微小RNA与先前描述的一组7种对CRC和CAA具有诊断预测性的微小RNA相结合。使用定量实时聚合酶链反应（qRT-PCR）评估微小RNA的差异表达。
纳入50例患者（n = 27例CRC，n = 23例CAA）。CRC患者与CAA患者在年龄、性别或种族方面无差异。CRC切除后6种微小RNA显著增加（miR-324、let-7b、miR-454、miR-374a、miR-122、miR-19b，均p<0.05），而CAA切除后3种微小RNA显著增加（miR-454、miR-374a、miR-122，均p<0.05）。两者共有3种微小RNA增加（miR-454、miR-374a、miR-122）。
与肿瘤形成相关的微小RNA（无论是CRC还是CAA）在手术切除或内镜切除CRC或CAA后表达显著增加。未来的研究应聚焦于评估这些微小RNA在CRC和CAA预后中的作用。
