Shenzhen Environmental Science and New Energy Technology Engineering Laboratory, Tsinghua-Berkeley Shenzhen Institute, Shenzhen 518055, PR China; Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, PR China.
Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, PR China.
Water Res. 2021 Feb 15;190:116710. doi: 10.1016/j.watres.2020.116710. Epub 2020 Nov 30.
Chlorination can lead to the formation of hazardous chlorinated disinfection byproducts (Cl-DBPs). We identified tyrosine (Tyr) and tryptophan (Trp) as precursors of toxic Cl-DBPs and developed a halogen extraction code to complement ultra performance liquid chromatography in tandem with high resolution mass spectrometry (UPLC-HRMS) in detecting and identifying Cl-DBPs. We detected 20 and 11 Cl-DBPs formed from chlorination of Tyr and Trp, respectively, and identified the structures of 15 Cl-DBPs. Fourteen structures were previously unreported. We also proposed the tentative formation pathways of these newly identified Cl-DBPs. Their incidence in real water sources demonstrated that these Cl-DBPs are likely to form during chlorination of reclaimed water. We computationally predicted the toxicity of these Cl-DBPs, which was relatively high, indicating that these Cl-DBPs could be hazardous and were of valid concern. Combining analytical data with the halogen extraction code can identify Cl-DBPs accurately from complex compounds. This analytical method can be used to identify Cl-DBPs of water treatment procedures in further studies.
氯化会导致形成有害的氯化消毒副产物（Cl-DBPs）。我们鉴定出酪氨酸（Tyr）和色氨酸（Trp）是有毒的 Cl-DBPs 的前体，并开发了一种卤代萃取码，以补充超高效液相色谱与高分辨率质谱联用（UPLC-HRMS）在检测和鉴定 Cl-DBPs 中的应用。我们分别检测到 Tyr 和 Trp 氯化生成的 20 种和 11 种 Cl-DBPs，并鉴定了 15 种 Cl-DBPs 的结构。其中 14 种结构以前未被报道过。我们还提出了这些新鉴定的 Cl-DBPs 的暂定形成途径。它们在实际水源中的存在表明，这些 Cl-DBPs 可能在再生水氯化过程中形成。我们通过计算预测了这些 Cl-DBPs 的毒性，发现它们的毒性相对较高，这表明这些 Cl-DBPs 可能具有危害性，值得关注。将分析数据与卤代萃取码相结合，可以从复杂化合物中准确地鉴定出 Cl-DBPs。这种分析方法可用于在进一步的研究中识别水处理程序中的 Cl-DBPs。
