Karlsruhe Institute of Technology (KIT), Engler-Bunte-Institut, Water Chemistry and Water Technology, Engler-Bunte-Ring 9a, 76131 Karlsruhe, Germany.
Karlsruhe Institute of Technology (KIT), Engler-Bunte-Institut, Water Chemistry and Water Technology, Engler-Bunte-Ring 9a, 76131 Karlsruhe, Germany; DVGW Research Centre at Engler-Bunte-Institut of Karlsruhe Institute of Technology (KIT), Water Chemistry, Engler-Bunte-Ring 9a, 76131 Karlsruhe, Germany.
Bioresour Technol. 2021 Feb;321:124444. doi: 10.1016/j.biortech.2020.124444. Epub 2020 Nov 26.
Biomethanation of CO has been proven to be a feasible way to produce methane with the employment of H as electron source. Subject of the present study is a custom-made membrane biofilm reactor for hydrogenotrophic methanation by archaeal biofilms cultivated on membrane surfaces. Reactor layout was adapted to allow for in situ biofilm analysis via optical coherence tomography. At a feeding ratio of H/CO of 3.6, and despite the low membrane surface to reactor volume ratio of 57.9 m m, the maximum methane production per reactor volume reached up to 1.17 Nm m d at a methane content of the produced gas above 97% (v/v). These results demonstrate that the concept of membrane bound biofilms enables improved mass transfer by delivering substrate gases directly to the biofilm, thus, rendering the bottleneck of low solubility of hydrogen in water less drastic.
已经证明，利用 H 作为电子源进行 CO 的生物甲烷化是生产甲烷的一种可行方法。本研究的主题是一种定制的膜生物膜反应器，用于在膜表面培养的古细菌生物膜进行氢营养型甲烷化。反应器的设计适应了通过光学相干断层扫描进行原位生物膜分析。在 H/CO 的进料比为 3.6 的情况下，尽管膜表面积与反应器体积比仅为 57.9 mm，但是在产生的气体中甲烷含量超过 97%（v/v）的情况下，每单位反应器体积的最大甲烷产量达到了 1.17 Nm m d。这些结果表明，通过将底物气体直接输送到生物膜，膜结合生物膜的概念能够实现更好的传质，从而降低了水中氢气低溶解度的瓶颈影响。
