School of Health Sciences, Lindenwood University, Saint Charles, MO 63301, USA.
School of Kinesiology, Auburn University, Auburn, AL 36849, USA.
Nutrients. 2020 Dec 3;12(12):3727. doi: 10.3390/nu12123727.
There is evidence in rodents to suggest that theacrine-based supplements modulate tissue sirtuin activity as well as other biological processes associated with aging. Herein, we examined if a theacrine-based supplement (termed NAD3) altered sirtuin activity in vitro while also affecting markers of mitochondrial biogenesis. The murine C2C12 myoblast cell line was used for experimentation. Following 7 days of differentiation, myotubes were treated with 0.45 mg/mL of NAD3 (containing ~2 mM theacrine) for 3 and 24 h ( = 6 treatment wells per time point). Relative to control (CTL)-treated cells, NAD3 treatments increased ( < 0.05) Sirt1 mRNA levels at 3 h, as well as global sirtuin activity at 3 and 24 h. Follow-up experiments comparing 24 h NAD3 or CTL treatments indicated that NAD3 increased nicotinamide phosphoribosyltransferase (NAMPT) and SIRT1 protein levels ( < 0.05). Cellular nicotinamide adenine dinucleotide (NAD) levels were also elevated nearly two-fold after 24 h of NAD3 versus CTL treatments ( < 0.001). Markers of mitochondrial biogenesis were minimally affected. Although these data are limited to select biomarkers in vitro, these preliminary findings suggest that a theacrine-based supplement can modulate select biomarkers related to NAD biogenesis and sirtuin activity. However, these changes did not drive increases in mitochondrial biogenesis. While promising, these data are limited to a rodent cell line and human muscle biopsy studies are needed to validate and elucidate the significance of these findings.
有证据表明，茶氨酸基补充剂可以调节组织中的沉默调节蛋白活性以及与衰老相关的其他生物过程。在此，我们研究了一种茶氨酸基补充剂（称为 NAD3）是否会改变体外沉默调节蛋白的活性，同时还会影响线粒体生物发生的标志物。使用鼠 C2C12 成肌细胞系进行实验。分化 7 天后，用 0.45 mg/mL 的 NAD3（含有~2 mM 茶氨酸）处理肌管 3 和 24 h（每个时间点有 6 个处理孔）。与对照（CTL）处理的细胞相比，NAD3 处理在 3 h 时增加了（<0.05）Sirt1 mRNA 水平，并且在 3 和 24 h 时增加了整体沉默调节蛋白活性。比较 24 h NAD3 或 CTL 处理的后续实验表明，NAD3 增加了烟酰胺磷酸核糖转移酶（NAMPT）和 SIRT1 蛋白水平（<0.05）。与 CTL 处理相比，细胞内烟酰胺腺嘌呤二核苷酸（NAD）水平在 NAD3 处理 24 h 后也升高了近两倍（<0.001）。线粒体生物发生的标志物受到的影响很小。尽管这些数据仅限于体外的一些生物标志物，但这些初步发现表明，茶氨酸基补充剂可以调节与 NAD 生物发生和沉默调节蛋白活性相关的一些生物标志物。然而，这些变化并没有导致线粒体生物发生的增加。虽然有希望，但这些数据仅限于一种啮齿动物细胞系，需要进行人类肌肉活检研究来验证和阐明这些发现的意义。
