Saxén L, Lehtonen E
Department of Pathology, University of Helsinki, Finland.
Differentiation. 1987;36(1):2-11. doi: 10.1111/j.1432-0436.1987.tb00176.x.
Organotypic cultures can be used for cultivating embryonic kidney rudiments either intact or dissociated into two interacting tissue components. This allows micromanipulations that would hardly be possible in vivo. Especially beneficial is the transfilter technique in which the interacting components are cultured on the opposite sides of a porous filter membrane. This model system allows precise timing of inductive interaction and temporal correlation of various developmental events. On the other hand, the manipulations and the in vitro conditions seem to cause metabolic changes, and long-term cultivation will result in damage to the tissues due to insufficient nutrition. Induced mesenchymal cells cultivated in monolayer cultures behave differently from those in three-dimensional organotypic explants: in the former, differentiation is largely impaired whereas in the latter, development closely resembles that in vivo. Although an organ culture system can never fully mimic embryogenesis in vivo, it offers advantages over cell cultures.
器官型培养可用于培养完整的胚胎肾原基，也可将其解离为两个相互作用的组织成分。这使得在体内几乎不可能进行的显微操作成为可能。特别有益的是跨滤器技术，其中相互作用的成分在多孔滤膜的相对两侧进行培养。该模型系统允许精确控制诱导相互作用的时间以及各种发育事件的时间相关性。另一方面，操作和体外条件似乎会引起代谢变化，长期培养会因营养不足而导致组织受损。单层培养中诱导的间充质细胞与三维器官型外植体中的细胞表现不同：在前者中，分化在很大程度上受到损害，而在后者中，发育与体内情况非常相似。尽管器官培养系统永远无法完全模拟体内胚胎发生，但它比细胞培养具有优势。
