胚胎肾原基和分离的输尿管芽的体外培养。
In vitro culture of embryonic kidney rudiments and isolated ureteric buds.
作者信息
Zhang Xing, Bush Kevin T, Nigam Sanjay K
机构信息
Pediatrics Department, University of California, San Diego, CA, USA.
出版信息
Methods Mol Biol. 2012;886:13-21. doi: 10.1007/978-1-61779-851-1_2.
PMID:22639247
Abstract
In vitro culture of embryonic kidney rudiments has been utilized to study a variety of cellular processes and developmental mechanisms. Here, we describe two-dimensional (2D) culture of embryonic kidney rudiments on Transwell filters and three-dimensional (3D) cultures in collagen gels in detail, and 3D culture of isolated ureteric bud (UB) in Matrigel with BSN-conditioned media.
摘要
胚胎肾原基的体外培养已被用于研究多种细胞过程和发育机制。在此，我们详细描述了胚胎肾原基在Transwell滤器上的二维（2D）培养以及在胶原凝胶中的三维（3D）培养，以及在基质胶中使用BSN条件培养基对分离的输尿管芽（UB）进行的三维培养。
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