Gallardo Paffetti María, Cárcamo Juan, Arias-Darraz Luis, Alvear Carlos, Ojeda Javier
Escuela de Medicina Veterinaria, Facultad de Ciencias, Universidad Mayor, Santiago PO Box 8580745, Chile.
Centro FONDAP, Interdisciplinary Center for Aquaculture Research (INCAR), Facultad de Ciencias, Universidad Austral de Chile, Valdivia PO Box 567, Chile.
Animals (Basel). 2020 Dec 3;10(12):2290. doi: 10.3390/ani10122290.
The following study was performed in order to determine the effect of type of pregnancy on the transcriptional expression of genes that are engaged in angiogenesis and cell turnover/lactogenesis in the ewe mammary gland, evaluating its impact on the plasma metabolic response. In addition, an assessment of its further influence on plasma metabolic response, performance, and muscle transcriptional expression of lipogenic enzymes in progeny lambs was made. Thirteen Ile de France sheep (six twin- and seven single-bearing ewes) were allocated to graze ad libitum naturalized pasture from d 45 pre-partum to day 70 post-partum, while keeping their lambs on the same diet until day 60 after weaning. The samples were collected at different times and analyzed by qRT-PCR and plasma metabolic indicators. The data were processed using SPSS package. The results showed that twin-bearing ewes overexpressed VEGFR1 at birth, and BCL2 at birth and day 35 post-partum; however, single-bearing ewes overexpressed CAIV and IGF1 at day 35 post-partum. Similar metabolite concentrations in blood plasma were found between groups of ewes. The plasma metabolic response in lambs was similar between groups and it did not influence their performance, where a similar transcriptional expression of lipogenic enzymes in muscle was observed. Therefore, the type of pregnancy can explain the slight differences in mRNA expression that were found in angiogenesis and cell turnover/lactogenesis in mammary gland, although these differences not only did not affect the plasma metabolic response in ewes, but they also had no influence on plasma metabolic response, performance, and muscle transcriptional expression of their lambs.
进行以下研究是为了确定妊娠类型对参与母羊乳腺血管生成和细胞更新/泌乳的基因转录表达的影响，评估其对血浆代谢反应的影响。此外，还评估了其对后代羔羊血浆代谢反应、生产性能以及脂肪生成酶肌肉转录表达的进一步影响。13只法兰西岛绵羊（6只怀有双胞胎的母羊和7只怀有单胞胎的母羊）被分配从产前45天到产后70天自由采食天然牧场，同时让它们的羔羊保持相同饮食直至断奶后60天。在不同时间采集样本，并通过qRT-PCR和血浆代谢指标进行分析。数据使用SPSS软件包进行处理。结果表明，怀有双胞胎的母羊在出生时VEGFR1过表达，在出生时和产后35天BCL2过表达；然而，怀有单胞胎的母羊在产后35天CAIV和IGF1过表达。在母羊组之间发现血浆中代谢物浓度相似。羔羊组之间的血浆代谢反应相似，且不影响它们的生产性能，在肌肉中观察到脂肪生成酶的转录表达相似。因此，妊娠类型可以解释在乳腺血管生成和细胞更新/泌乳中发现的mRNA表达的细微差异，尽管这些差异不仅没有影响母羊的血浆代谢反应，而且对其羔羊的血浆代谢反应、生产性能和肌肉转录表达也没有影响。
