Shimazu S
Department of Surgery, Kitasato University School of Medicine, Sagamihara, Japan.
Nihon Geka Gakkai Zasshi. 1987 Dec;88(12):1667-75.
In this experiment, wound fluid (WF) was obtained by subcutaneous implantation of a stainless steel mesh cylinder through a 7 cm incision in the dorsal skin of Lewis male rats. On the tenth day after implantation, the fluid in the cylinder was aspirated. The rats in the three groups were studied after receiving dermal allografts from ACI rats. The group which received daily intraperitoneal injections of WF was found to have a graft survival time twice as long as the control groups. Two other groups were studied after receiving dermal xenografts of lyophilized porcine dermis. Until the seventh day the grafts remained elastic but then began to show signs of rejection. There was no difference in graft survival time between the group receiving WF injections and the control group. Although burn wound fluid was found to have a level of PGE 139 times normal, fluid obtained from a 7 cm skin incision on the tenth post-wounding day did not have a dramatic increase in PGE. WF appears to prolong survival of allografts but not xenografts; It is possible that PGE does not play a significant role in immunosuppressive properties of WF.
在本实验中，通过在雄性Lewis大鼠背部皮肤做一个7厘米的切口，皮下植入一个不锈钢网圆柱体来获取伤口渗出液（WF）。植入后第十天，吸出圆柱体内的液体。三组大鼠在接受来自ACI大鼠的皮肤同种异体移植后进行研究。发现每天腹腔注射WF的组，其移植物存活时间是对照组的两倍。另外两组在接受冻干猪真皮的皮肤异种移植后进行研究。直到第七天，移植物仍保持弹性，但随后开始出现排斥迹象。接受WF注射的组与对照组之间的移植物存活时间没有差异。尽管发现烧伤伤口渗出液中PGE的水平是正常水平的139倍，但在受伤后第十天从7厘米皮肤切口获取的液体中，PGE并没有显著增加。WF似乎能延长同种异体移植物的存活时间，但不能延长异种移植物的存活时间；PGE在WF的免疫抑制特性中可能不发挥重要作用。
