Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 239 Zhangheng Road, Pudong New District, Shanghai 201203, China.
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, No. 2019 Jia Luo Road, Jiading District, Shanghai 201800, China.
Anal Chem. 2021 Jan 19;93(2):671-676. doi: 10.1021/acs.analchem.0c04110. Epub 2020 Dec 8.
The central relevance of cellular heterogeneity to biological phenomena raises the rational needs for analytical techniques with single-cell resolution. Here, we developed a single-cell FTIR microspectroscopy-based method for the quantitative evaluation of cellular heterogeneity by calculating the cell-to-cell similarity distance of the infrared spectral data. Based on this method, we revealed the infrared phenotypes might reflect the dynamic heterogeneity changes in the cell population during the adipogenic differentiation of the human mesenchymal stem cells. These findings provide an alternative label-free optical approach for quantifying the cellular heterogeneity, and the combination with other single-cell analysis tools will be very helpful for understanding the genotype-to-phenotype relationship in cellular populations.
细胞异质性对生物现象的核心相关性提出了对具有单细胞分辨率的分析技术的合理需求。在这里，我们开发了一种基于单细胞傅里叶变换红外（FTIR）显微光谱的方法，通过计算红外光谱数据的细胞间相似距离，对细胞异质性进行定量评估。基于该方法，我们揭示了红外表型可能反映了人骨髓间充质干细胞成脂分化过程中细胞群体的动态异质性变化。这些发现为量化细胞异质性提供了一种替代的无标记光学方法，与其他单细胞分析工具相结合将非常有助于理解细胞群体中的基因型-表型关系。
