Zemek Rostislav, Konopická Jana, Ul Abdin Zain
Arthropod Ecology and Biological Control Research Group, Ton Duc Thang University, Ho Chi Minh City 758307, Vietnam.
Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City 758307, Vietnam.
J Fungi (Basel). 2020 Dec 6;6(4):342. doi: 10.3390/jof6040342.
sp. is an important native and ornamental tree in Europe threatened by a serious invasive pest . The larvae of this moth are able to defoliate box trees and cause their death. The development of novel biopesticides targeting this pest might help protect trees grown wildly or in city parks. Laboratory experiments were conducted to assess the efficacy of entomopathogenic fungus strain CCM 8367 against . The last-instar larvae of the box tree moth were treated by the suspension of fungus conidia at concentrations ranging from 1 × 10 to 1 × 10 spores per 1 mL. Fungus infection was observed mostly in pupae, but the maximum mortality did not exceed 60%, indicating a very low susceptibility of to . Furthermore, a number of ungerminated fungal conidia were found on larval cuticles using a low-temperature scanning electron microscopy. Our data also reveal that the hydroalcoholic extract from leaves significantly inhibits both the germination of conidia and fungus growth. It can be speculated that the strain CCM 8367 of is not a potent biocontrol agent against and low virulence of the fungus might be due to the accumulation of host plant phytochemicals having antimicrobial activity in larval cuticle of the pest.
某树种是欧洲一种重要的本土观赏树木，受到一种严重入侵害虫的威胁。这种蛾的幼虫能够使黄杨木落叶并导致其死亡。开发针对这种害虫的新型生物农药可能有助于保护野生或城市公园中种植的树木。进行了实验室实验以评估昆虫病原真菌菌株CCM 8367对该害虫的防治效果。用浓度为每1毫升1×10到1×10个孢子的真菌分生孢子悬浮液处理黄杨绢野螟的末龄幼虫。真菌感染主要在蛹期观察到，但最高死亡率不超过60%，表明该害虫对该真菌的易感性非常低。此外，使用低温扫描电子显微镜在幼虫表皮上发现了许多未萌发的真菌分生孢子。我们的数据还表明，该树种叶片的水醇提取物显著抑制该真菌分生孢子的萌发和真菌生长。可以推测，该真菌的CCM 8367菌株不是防治该害虫的有效生物防治剂，真菌的低毒力可能是由于害虫幼虫表皮中具有抗菌活性的寄主植物植物化学物质的积累。
