Vega FE, Dowd PF, McGuire MR, Jackson MA, Nelsen TC
Agricultural Research Service, National Center for Agricultural Utilization Research, USDA, 1815 North University Street, Peoria, Illinois, 61604
J Invertebr Pathol. 1997 Nov;70(3):209-13. doi: 10.1006/jipa.1997.4693.
Seven secondary plant compounds (catechol, chlorogenic acid, gallic acid, salicylic acid, saponin, sinigrin, and tannic acid) mixed with Noble agar at three concentrations (100, 500, and 1000 ppm) were tested for their effects on germination of blastospores of the fungal entomopathogen Paecilomyces fumosoroseus. With individual allelochemicals incorporated at 100 ppm in Noble agar, significant differences in time to 95% germination were found between two allelochemicals (catechol and salicylic acid) and the control. Blastospores in media containing 100 ppm catechol took twice as long (10 hr) to reach 95% germination as the control. Germination of blastospores in medium containing catechol, salicylic acid, or tannic acid at 500 was 55, 56, and 46%, respectively, in contrast to less than 10% when the concentration was 1000 ppm. These results indicate that the presence of allelochemicals on a substrate (e.g., insect cuticle or leaf) may be an additional constraint to the survival of entomopathogenic fungi. Copyright 1997 Academic Press. Copyright 1997 Academic Press
将七种次生植物化合物（儿茶酚、绿原酸、没食子酸、水杨酸、皂苷、芥子苷和单宁酸）与诺布尔琼脂以三种浓度（100、500和1000 ppm）混合，测试它们对真菌昆虫病原体玫烟色拟青霉芽生孢子萌发的影响。在诺布尔琼脂中加入100 ppm的单一化感物质时，发现两种化感物质（儿茶酚和水杨酸）与对照之间在达到95%萌发所需时间上存在显著差异。含有100 ppm儿茶酚的培养基中的芽生孢子达到95%萌发所需时间是对照的两倍（10小时）。在含有500 ppm儿茶酚、水杨酸或单宁酸的培养基中，芽生孢子的萌发率分别为55%、56%和46%，而浓度为1000 ppm时萌发率低于10%。这些结果表明，底物（如昆虫表皮或叶片）上化感物质的存在可能是对昆虫病原真菌生存的额外限制。版权所有1997年学术出版社。版权所有1997年学术出版社
