Biomedical Translation Research Center, Academia Sinica, Taipei 115, Taiwan.
Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan.
Cells. 2020 Dec 4;9(12):2610. doi: 10.3390/cells9122610.
Magnetic resonance imaging (MRI) is extensively used in clinical and basic biomedical research. However, MRI detection of pH changes still poses a technical challenge. Chemical exchange saturation transfer (CEST) imaging is a possible solution to this problem. Using saturation transfer, alterations in the exchange rates between the solute and water protons because of small pH changes can be detected with greater sensitivity. In this study, we examined a fatigued skeletal muscle model in electrically stimulated mice. The measured CEST signal ratio was between 1.96 ppm and 2.6 ppm in the z-spectrum, and this was associated with pH values based on the ratio between the creatine (Cr) and the phosphocreatine (PCr). The CEST results demonstrated a significant contrast change at the electrical stimulation site. Moreover, the pH value was observed to decrease from 7.23 to 7.15 within 20 h after electrical stimulation. This pH decrease was verified by P magnetic resonance spectroscopy and behavioral tests, which showed a consistent variation over time.
磁共振成像（MRI）在临床和基础生物医学研究中得到了广泛应用。然而，MRI 检测 pH 值变化仍然存在技术挑战。化学交换饱和转移（CEST）成像是解决这个问题的一种可能方法。通过饱和转移，可以更敏感地检测到由于 pH 值微小变化而导致的溶质和水质子之间交换率的变化。在这项研究中，我们检查了电刺激小鼠的疲劳骨骼肌模型。在 z 谱中，CEST 信号比在 1.96 ppm 和 2.6 ppm 之间，并且基于肌酸（Cr）和磷酸肌酸（PCr）之间的比率与 pH 值相关。CEST 结果显示在电刺激部位有明显的对比度变化。此外，在电刺激后 20 小时内，pH 值从 7.23 下降到 7.15。通过 P 磁共振波谱和行为测试验证了这种 pH 值下降，这些测试显示出随时间的一致变化。
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