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镉胁迫下细胞蛋白质组学分析数据集。

Dataset for proteomic analysis of cells under cadmium stress.

作者信息

León-Vaz Antonio, Romero Luis C, Gotor Cecilia, León Rosa, Vigara Javier

机构信息

Laboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and REMSMA. University of Huelva, 210071 Huelva, Spain.

Instituto de Bioquímica Vegetal y Fotosíntesis. Consejo Superior de Investigaciones Científicas and Universidad de Sevilla. Avenida Américo Vespucio, 49. 41092 Seville. Spain.

出版信息

Data Brief. 2020 Nov 18;33:106544. doi: 10.1016/j.dib.2020.106544. eCollection 2020 Dec.

Abstract

Cadmium is one of the most hazardous heavy metal for aquatic environments and one of the most toxic contaminants for phytoplankton. This work provides the dataset associated with the research publication "Effect of cadmium in the microalga : a proteomic study" [1]. This dataset describes a proteomic approach, based on the sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS), derived from exposure of to 250 µM Cd for 40 h, showing the proteins that are up- or downregulated. The processing of data included the identification of the protein sequences equivalent to the corresponding of sequences obtained, which made possible to use KEGG Database. MS and MS/MS information, and quantitative data were deposited PRIDE public repository under accession number PXD015932.

摘要

镉是对水生环境危害最大的重金属之一,也是对浮游植物毒性最强的污染物之一。这项工作提供了与研究论文《镉对微藻的影响:蛋白质组学研究》[1]相关的数据集。该数据集描述了一种基于所有理论碎片离子谱质谱(SWATH-MS)的序列窗口采集的蛋白质组学方法,该方法源自将微藻暴露于250µM镉中40小时,展示了上调或下调的蛋白质。数据处理包括鉴定与所获得的相应序列等同的蛋白质序列,这使得使用KEGG数据库成为可能。质谱和串联质谱信息以及定量数据已存入PRIDE公共储存库,登录号为PXD015932。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e9c/7695938/21d1ea038384/gr1.jpg

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