Nakashima J, Hasegawa S, Nakashima Y, Nakamura S, Kimura S, Tachibana M, Deguchi N, Tazaki H
Department of Urology, Second Tokyo National Hospital.
Hinyokika Kiyo. 1987 Dec;33(12):2059-63.
Localization of gamma-seminoprotein (gamma-Sm) was examined using horseradish peroxidase-labeled anti-gamma-Sm antibody (Chugai Corp. Ltd., Tokyo, Japan) by the enzyme-labeled antibody method in paraffin embedded specimens of 18 benign prostatic hyperplasias and 32 untreated prostatic cancers. The level of serum gamma-Sm was also determined by enzyme immunoassay in 10 untreated patients with prostatic cancer and 18 with benign prostatic hyperplasia. Specific gamma-Sm staining was detected in prostatic glandular epithelial cells and prostatic secretion of all specimens of benign prostatic hyperplasias and a half of the specimens of prostatic cancers. Specific gamma-Sm staining was shown to correlate with histological differentiation of the prostatic cancer, but no correlation was found between specific gamma-Sm staining and the level of serum gamma-Sm.
采用辣根过氧化物酶标记的抗γ-精浆蛋白（γ-Sm）抗体（日本东京中外制药株式会社），通过酶标抗体法对18例良性前列腺增生和32例未经治疗的前列腺癌石蜡包埋标本进行γ-精浆蛋白（γ-Sm）定位研究。同时采用酶免疫分析法检测了10例未经治疗的前列腺癌患者和18例良性前列腺增生患者的血清γ-Sm水平。在所有良性前列腺增生标本及一半的前列腺癌标本的前列腺腺上皮细胞和前列腺分泌物中检测到特异性γ-Sm染色。结果显示，特异性γ-Sm染色与前列腺癌的组织学分化相关，但特异性γ-Sm染色与血清γ-Sm水平之间未发现相关性。
