Hayashida N, Matsubayashi T, Shinozaki K, Sugiura M, Inoue K, Hiyama T
Center for Gene Research, Nagoya University, Japan.
Curr Genet. 1987;12(4):247-50. doi: 10.1007/BF00435285.
The gene for the 9 kd polypeptide (a possible apoprotein for the iron-sulfur centers A and B) of photosystem I has been located in the small single-copy region of tobacco chloroplast DNA. This gene (psaC) was identified by comparing the N-terminal amino acid sequence of the spinach 9 kd polypeptide with the entire sequence of tobacco chloroplast genome. The gene organization is ndhE (101 codons)--263 bp spacer--psaC (81 codons)--94 bp spacer--ndhD (509 codons). Northern blot hybridization revealed that psaC is transcribed in the chloroplasts. The deduced amino acid sequence and secondary structure are presented. The predicted polypeptide is rich in cysteine residues and contains a unique repeated sequence.
光系统I的9kd多肽（可能是铁硫中心A和B的脱辅基蛋白）的基因已定位在烟草叶绿体DNA的小单拷贝区域。通过将菠菜9kd多肽的N端氨基酸序列与烟草叶绿体基因组的全序列进行比较，鉴定出了该基因（psaC）。基因组织为ndhE（101个密码子）-263bp间隔区-psaC（81个密码子）-94bp间隔区-ndhD（509个密码子）。Northern印迹杂交表明psaC在叶绿体中被转录。给出了推导的氨基酸序列和二级结构。预测的多肽富含半胱氨酸残基，并包含一个独特的重复序列。
