Department of Chemistry, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
Department of Chemistry, Faculty of Mathematics and Sciences, Universitas Indonesia, Kampus UI Depok, Jakarta 16-4424, Indonesia.
Anal Chem. 2021 Feb 2;93(4):2336-2341. doi: 10.1021/acs.analchem.0c04212. Epub 2020 Dec 9.
The electrogenerated chemiluminescence of luminol is a process by which light generation is triggered by adding hydrogen peroxide and then applying a suitable electrode potential. Here, we take this phenomenon one step forward by avoiding the addition of hydrogen peroxide using a smart combination of a boron-doped diamond electrode and a carbonate electrolyte to generate the hydrogen peroxide directly in situ. The reaction occurs because of the carbonate electrochemical oxidation to peroxydicarbonate and the following hydrolysis to hydrogen peroxide, which triggers the emission from luminol. The electrogenerated chemiluminescence emission has been optimized by an investigation of the applied potentials, the carbonate concentration, and the pH. Furthermore, these results have been used to shine a light on the reaction mechanisms. Because this method does not require the addition of hydrogen peroxide, it might find application in efforts to avoid instability of hydrogen peroxide or its interference with the analytes of interest.
鲁米诺的电致化学发光是一种通过添加过氧化氢并施加适当电极电势来触发发光的过程。在这里，我们通过巧妙地结合掺硼金刚石电极和碳酸盐电解质来避免添加过氧化氢，从而直接原位生成过氧化氢，将这一现象向前推进了一步。由于碳酸盐的电化学氧化生成过氧二碳酸酯以及随后的水解生成过氧化氢，反应发生，从而触发鲁米诺的发光。通过研究施加的电势、碳酸盐浓度和 pH 值，优化了电致化学发光的发射。此外，这些结果还用于揭示反应机制。由于这种方法不需要添加过氧化氢，因此它可能适用于避免过氧化氢的不稳定性或其对感兴趣的分析物的干扰。
