Meucci Valentina, Battaglia Federica, Marchetti Veronica, Gori Eleonora, Intorre Luigi
Department of Veterinary Science, University of Pisa, 56122 Via Livornese, San Piero a Grado, Pisa, Italy.
MethodsX. 2020 Nov 20;7:101154. doi: 10.1016/j.mex.2020.101154. eCollection 2020.
The conventional analytical techniques used for the quantitative analysis of heavy metals in animal tissues are the atomic absorption spectroscopy (AAS) and the inductively-coupled plasma mass spectrometry (ICP-MS). These methods involve high cost, skilled personnel and long analysis times. Several studies have shown the applicability of electrochemical transducer coupled to disposable screen-printed electrodes (SPE) for measuring metals. The aim of the present study was to applicate and validate a simple and fast protocol for the simultaneous measurement of Cu and Pb concentrations in canine liver biopsy, coupling a simple digestion procedure with electrochemical stripping analysis. Square wave anodic stripping voltammetry (SWASV) coupled to disposable SPE was employed as fast and sensitive method for metals detection. Samples were digested with hydrogen peroxide/hydrochloric acid mixture for 1 h coupled to solid phase purification with carbon columns. Instrumental precision, digestion procedure accuracy and limits of detections were evaluated. To validate the proposed method the metal concentrations have also been determined by AAS. Instrumental precision and digestion method accuracy were less than 15.3% and 16.8% for Cu and Pb, respectively. The limits of detection were 10 and 6 mg/kg dry weight in liver samples for Cu and Pb, respectively. The results obtained by the electrochemical method are in agreement with those of the reference method. The obtained results showed a reliable approach for Cu and Pb detection simultaneously with good sensitivity, accuracy and precision. The simple sample pre-treatment together with the low cost makes this approach particularly appealing for Cu and Pb quantification for diagnostic purposes.•Simultaneous determination of Cu and Pb concentrations in canine liver biopsy.•Square wave anodic stripping voltammetry (SWASV) with screen-printed electrodes (SPE) coupled to an acid digestion protocol.•Cu and Pb were measured with a very good sensitivity and accuracy.•Good agreement with AAS reference method.•Low cost, fast and sensitive method for diagnostic purposes.
用于动物组织中重金属定量分析的传统分析技术是原子吸收光谱法（AAS）和电感耦合等离子体质谱法（ICP-MS）。这些方法成本高、需要技术人员且分析时间长。多项研究表明，电化学传感器与一次性丝网印刷电极（SPE）联用可用于金属测量。本研究的目的是应用并验证一种简单快速的方案，用于同时测量犬肝活检组织中的铜和铅浓度，即将简单的消化程序与电化学溶出分析相结合。采用与一次性SPE联用的方波阳极溶出伏安法（SWASV）作为快速灵敏的金属检测方法。样品用双氧水/盐酸混合物消化1小时，再通过碳柱进行固相净化。评估了仪器精密度、消化程序准确性和检测限。为验证所提出的方法，还通过AAS测定了金属浓度。铜和铅的仪器精密度和消化方法准确性分别小于15.3%和16.8%。肝样品中铜和铅的检测限分别为10和6mg/kg干重。电化学方法获得的结果与参考方法的结果一致。所得结果表明，该方法是一种可靠的同时检测铜和铅的方法，具有良好的灵敏度、准确性和精密度。简单的样品预处理以及低成本使得该方法对于诊断目的的铜和铅定量特别有吸引力。
•同时测定犬肝活检组织中的铜和铅浓度。
•方波阳极溶出伏安法（SWASV）与丝网印刷电极（SPE）联用并结合酸消化程序。
•铜和铅的测量具有非常好的灵敏度和准确性。
•与AAS参考方法高度一致。
•用于诊断目的的低成本、快速且灵敏的方法。
