Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province, College of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, P. R. China.
State Key Laboratory of Electroanalytical Chemistry, Research Center of Biomembranomics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, P. R. China.
Anal Chem. 2021 Jan 19;93(2):936-945. doi: 10.1021/acs.analchem.0c03630. Epub 2020 Dec 10.
Protein tyrosine kinase-7 (PTK7), as an important membrane receptor, regulates various cellular activities, including cell polarity, movement, migration, and invasion. Although lots of research studies focused on revealing its functions from the aspect of the expression of the gene and protein are present, the relationship between the spatial distribution at the single-molecule level and the function remains unclear. Through combining aptamer probe labeling and super-resolution imaging technology, after verifying the specificity and superiority of the aptamer probe, a more significant clustering distribution of PTK7 is found on the MCF10A cell basal membrane than on the apical membrane, which is thought to be related to their specific functions on different membranes. By exploring the relationship between the assembly of PTK7 and lipid rafts, actin cytoskeleton, and carbohydrate chains on the membrane, the unique distribution of PTK7 on disparate membranes is revealed to be probably because of the varied dominant position of these three factors. These findings present the detailed spatial information of PTK7 and the related potential organization mechanism on the cell membrane, which will facilitate a better understanding of the relationship between the molecular assembly and its function, as well as the overall structure of the cell membrane.
蛋白酪氨酸激酶 7（PTK7）作为一种重要的膜受体，调节着细胞的各种活动，包括细胞极性、运动、迁移和侵袭。虽然有很多研究集中在从基因和蛋白质表达的角度揭示其功能，但空间分布在单分子水平上与功能之间的关系仍不清楚。通过结合适体探针标记和超分辨率成像技术，在验证了适体探针的特异性和优越性后，发现 PTK7 在 MCF10A 细胞基底膜上的聚集分布比在顶膜上更为显著，这可能与它们在不同膜上的特定功能有关。通过探索 PTK7 与膜脂筏、肌动蛋白细胞骨架和糖链的组装之间的关系，揭示了 PTK7 在不同膜上的独特分布可能是由于这三个因素的主导地位不同。这些发现提供了 PTK7 在细胞膜上的详细空间信息和相关的潜在组织机制，有助于更好地理解分子组装与其功能以及整个细胞膜的整体结构之间的关系。
