Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", via Vivaldi, 43, 81100 Caserta, Italy.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", via Vivaldi, 43, 81100 Caserta, Italy; Institute of Genetics and Biophysics "A. Buzzati-Traverso" - CNR, Via Pietro Castellino, 111, 80131 Naples, Italy.
Int J Biol Macromol. 2021 Jan 31;168:67-76. doi: 10.1016/j.ijbiomac.2020.12.029. Epub 2020 Dec 7.
ZBTB2 is a protein belonging to the BTB/POZ zinc-finger family whose members typically contain a BTB/POZ domain at the N-terminus and several zinc-finger domains at the C-terminus. Studies have been carried out to disclose the role of ZBTB2 in cell proliferation, in human cancers and in regulating DNA methylation. Moreover, ZBTB2 has been also described as an ARF, p53 and p21 gene repressor as well as an activator of genes modulating pluripotency. In this scenario, ZBTB2 seems to play many functions likely associated with other proteins. Here we report a picture of the ZBTB2 protein partners in U87MG cell line, identified by high-resolution mass spectrometry (MS) that highlights the interplay between ZBTB2 and chromatin remodeling multiprotein complexes. In particular, our analysis reveals the presence, as ZBTB2 candidate interactors, of SMARCA5 and BAZ1B components of the chromatin remodeling complex WICH and PBRM1, a subunit of the SWI/SNF complex. Intriguingly, we identified all the subunits of the NuRD complex among the ZBTB2 interactors. By co-immunoprecipitation experiments and ChIP-seq analysis we definitely identify ZBTB2 as a new partner of the NuRD complex.
ZBTB2 是一种属于 BTB/POZ 锌指家族的蛋白质，其成员通常在 N 端包含一个 BTB/POZ 结构域，在 C 端包含几个锌指结构域。已经进行了研究以揭示 ZBTB2 在细胞增殖、人类癌症和调节 DNA 甲基化中的作用。此外，ZBTB2 还被描述为 ARF、p53 和 p21 基因的抑制剂以及调节多能性的基因的激活剂。在这种情况下，ZBTB2 似乎发挥了许多可能与其他蛋白质相关的功能。在这里，我们通过高分辨率质谱 (MS) 报道了 U87MG 细胞系中 ZBTB2 蛋白伴侣的图片，该图片突出了 ZBTB2 与染色质重塑多蛋白复合物之间的相互作用。特别是，我们的分析揭示了作为 ZBTB2 候选相互作用物的染色质重塑复合物 WICH 的 SMARCA5 和 BAZ1B 成分以及 SWI/SNF 复合物的 PBRM1 亚基的存在。有趣的是，我们在 ZBTB2 相互作用物中鉴定出了 NuRD 复合物的所有亚基。通过共免疫沉淀实验和 ChIP-seq 分析，我们明确确定 ZBTB2 是 NuRD 复合物的一个新伴侣。
