McKeever P E, Spicer S S, Brissie N T, Garvin A J
J Histochem Cytochem. 1977 Sep;25(9):1063-8. doi: 10.1177/25.9.333019.
Receptors for immune complexes have been localized on rabbit alveolar macrophages with scanning electron microscopy by exposing the cells first to a soluble immune complex composed of horseradish peroxidase and antibody to horseradish peroxidase, and then incubating with a benzidine-containing substrate that yields crystalline reaction product. Receptors were visualized by this means as sites of attachment of laminated slender crystals that were easily distinguished from macrophage surface structures. Receptors appeared most abundant on cytoplasmic veils and pseudopods and in the perinuclear region of macrophages minimally spread over the coverslip. Further macrophage spreading was associated with lighter receptor staining.
通过扫描电子显微镜，将兔肺泡巨噬细胞先暴露于由辣根过氧化物酶和抗辣根过氧化物酶抗体组成的可溶性免疫复合物中，然后与产生结晶反应产物的含联苯胺底物一起孵育，从而将免疫复合物受体定位在兔肺泡巨噬细胞上。通过这种方式，受体可被视为层状细长晶体附着的部位，这些晶体很容易与巨噬细胞表面结构区分开来。受体在细胞质膜和伪足以及巨噬细胞的核周区域最为丰富，这些巨噬细胞在盖玻片上的铺展程度最小。巨噬细胞进一步铺展与受体染色变浅有关。
