College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, PR China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, PR China.
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, PR China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, PR China.
Food Chem. 2021 May 30;345:128655. doi: 10.1016/j.foodchem.2020.128655. Epub 2020 Nov 19.
The aim of this study was to determine the influence and mechanism of combining EGCG with TGase on properties of myofibrillar protein (MP) gel. A double-crosslinked effect was observed when EGCG and TGase were added into MP gel. Breaking force, deformation, water holding capacity and hardness of double-crosslinked MP gel increased by 25.3 ± 3.0 g, 0.5 ± 0.3 mm, 1.76 ± 0.4% and 34.11 ± 2.56 g, compared with those of TGase induced gel. Light microscopy and low-field nuclear magnetic resonance results indicated with EGCG content increasing, pores and structure of double-crosslinked gels became smaller and denser, T decreased from 266.162 ms to 252.845 ms and its proportion increased from 94.103% to 96.956%. Molecular docking illustrated covalent and non-covalent interactions between EGCG and myosin heavy chain Ⅱ A, and confirmed TGase catalytic mechanism with myosin heavy chain Ⅱ A as substrate. Therefore the mixture of EGCG and TGase could be used as novel cross-linker in surimi.
本研究旨在确定 EGCG 与 TGase 联合对肌原纤维蛋白（MP）凝胶性质的影响及其机制。当 EGCG 和 TGase 添加到 MP 凝胶中时，观察到双交联效应。与 TGase 诱导的凝胶相比，双交联 MP 凝胶的断裂力、变形、持水力和硬度分别增加了 25.3±3.0g、0.5±0.3mm、1.76±0.4%和 34.11±2.56g。光镜和低场核磁共振结果表明，随着 EGCG 含量的增加，双交联凝胶的孔隙和结构变得更小、更致密，T 从 266.162ms 降低到 252.845ms，其比例从 94.103%增加到 96.956%。分子对接表明 EGCG 与肌球蛋白重链Ⅱ A 之间存在共价和非共价相互作用，并证实了 TGase 以肌球蛋白重链Ⅱ A 为底物的催化机制。因此，EGCG 和 TGase 的混合物可作为一种新型的鱼糜交联剂。
