School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China.
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China.
Food Chem. 2021 May 30;345:128817. doi: 10.1016/j.foodchem.2020.128817. Epub 2020 Dec 7.
Here, an efficient method for synthesizing carbon dots (CDs) using a deep eutectic solvent (DES) was developed. To investigate the influence of different DESs on the quantum yield of CDs, different hydrogen-bonding acceptors (HBAs) and hydrogen-bonding donors (HBDs) were used to synthesize the DES and prepare CDs. Using Sophora flavescens Aiton as precursor, CDs were prepared using choline chloride (ChCl)/urea based DES as reaction media and doping agent in the presence of water. The CDs showed strong blue fluorescence and were further modified with polyethyleneimine (CDs@PEI). The fluorescence intensity of CDs@PEI was selectively quenched by myricetin with a limit of detection (LOD) of 10 nM. Furthermore, CDs@PEI was used to analyze myricetin in the extracts that were fluorescent by DES with satisfactory performance of Abelmoschus manihot (Linn.) Medicus flowers, vine teas and blueberries. Finally, the bio-imaging application of CDs@PEI was tested and the results confirmed its potential application in bio-imaging.
这里开发了一种使用深共熔溶剂（DES）合成碳点（CDs）的有效方法。为了研究不同 DES 对 CDs 量子产率的影响，使用了不同的氢键供体（HBD）和氢键受体（HBA）来合成 DES 并制备 CDs。以苦参（Sophora flavescens Aiton）为前驱体，在水存在的条件下，以氯化胆碱（ChCl）/尿素为反应介质和掺杂剂，制备 CDs。CDs 呈现出强的蓝色荧光，并进一步用聚乙烯亚胺（PEI）进行修饰。PEI 修饰的 CDs（CDs@PEI）对杨梅素表现出选择性荧光猝灭，检测限（LOD）为 10 nM。此外，CDs@PEI 被用于分析 DES 荧光提取物中的杨梅素，对蕹菜、藤茶和蓝莓具有令人满意的分析性能。最后，测试了 CDs@PEI 的生物成像应用，结果证实了其在生物成像中的潜在应用。
