Cui Tianqi, Fan Ya, Liu Yaping, Ding Yangyue, Li Xinyue, Cheng Guiguang, Cheng Jianjun
Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650550, China.
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Foods. 2023 Dec 22;13(1):58. doi: 10.3390/foods13010058.
This study aimed to synthesize antibacterial carbon quantum dots (SP-CDs) from polyethyleneimine and spermidine via hydrothermal reaction. It was revealed that SP-CDs, with small size (7.18 nm) and high positive charge (+31.15 mV), had good fluorescence properties and lots of amino groups on their surfaces. The inhibition effect of SP-CDs on was better than that towards , and the SP-CDs also had an inhibitory effect on multi-drug-resistant . The mechanism of SP-CDs shows that the SP-CDs were adsorbed on the surface of the negatively charged cell membrane through electrostatic interaction. SP-CDs can cause changes in membrane permeability, resulting in a shift of the cell membrane from order to disorder and the decomposition of chemical components, followed by the leakage of cell contents, resulting in bacterial death. SP-CDs can also significantly inhibit biofilm formation, destroy mature biofilms and reduce the number of living cells. Moreover, SP-CDs had negligible antimicrobial resistance even after 18 generations of treatment. This study proves that SP-CDs effectively inhibit the proliferation of foodborne pathogens, providing new feasibility for the application of carbon-based nanomaterials in the food industry.
本研究旨在通过水热反应由聚乙烯亚胺和亚精胺合成抗菌碳量子点（SP-CDs）。结果表明，SP-CDs尺寸小（7.18纳米）且带高正电荷（+31.15毫伏），具有良好的荧光性能且表面有大量氨基。SP-CDs对[此处原文缺失具体细菌名称]的抑制效果优于对[此处原文缺失具体细菌名称]的抑制效果，并且SP-CDs对多重耐药菌也有抑制作用。SP-CDs的作用机制表明，SP-CDs通过静电相互作用吸附在带负电荷的细胞膜表面。SP-CDs可引起膜通透性改变，导致细胞膜从有序变为无序以及化学成分分解，随后细胞内容物泄漏，导致细菌死亡。SP-CDs还可显著抑制生物膜形成，破坏成熟生物膜并减少活细胞数量。此外，即使经过18代处理，SP-CDs的抗菌耐药性也可忽略不计。本研究证明SP-CDs有效抑制食源性病原体的增殖，为碳基纳米材料在食品工业中的应用提供了新的可行性。
