Laboratory of Optimization, Design, and Advanced Control - Fermentation Division (LOPCA-Ferm), School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil.
The Ohio State University, Department of Animal Sciences, Ohio State Agricultural Research and Development Center, Wooster, OH, USA.
Bioresour Technol. 2021 Feb;321:124504. doi: 10.1016/j.biortech.2020.124504. Epub 2020 Dec 7.
To enable the production of butanol with undiluted, non-detoxified sugarcane bagasse hemicellulose hydrolysates, this study developed a three-staged repeated-batch immobilized cell fermentation in which the efficiency of a 3D-printed nylon carrier to passively immobilize Clostridium saccharoperbutylacetonicum DSM 14923 was compared with sugarcane bagasse. The first stage consisted of sugarcane molasses fermentation, and in the second stage, non-detoxified sugarcane bagasse hemicellulose hydrolysates (SBHH) was pulse-fed to sugarcane molasses fermentation. In the next four batches, immobilized cells were fed with undiluted SBHH supplemented with molasses, and SBHH-derived xylose accounted for approximately 50% of the sugars. Bagasse was a superior carrier, and the average xylose utilization (33%) was significantly higher than the treatment with the 3D-printed carrier (16%). Notably, bagasse allowed for 43% of the butanol to be SBHH-derived. Overall, cell immobilization on lignocellulosic materials can be an efficient strategy to produce butanol from repeated-batch fermentation of non-detoxified hemicellulose hydrolysates.
为了能够利用未经稀释、未经解毒的甘蔗渣半纤维素水解物生产丁醇，本研究开发了一种三阶段重复分批固定化细胞发酵方法，比较了 3D 打印尼龙载体和甘蔗渣被动固定化丙酮丁醇梭菌（DSM 14923）的效率。第一阶段为甘蔗蜜发酵，第二阶段为脉冲式进料非解毒甘蔗渣半纤维素水解物（SBHH）至甘蔗蜜发酵。在下四个批次中，固定化细胞用未稀释的 SBHH 补加蜜饯喂养，SBHH 衍生的木糖约占糖的 50%。蔗渣是一种较好的载体，木糖利用率（33%）明显高于 3D 打印载体处理（16%）。值得注意的是，蔗渣使 43%的丁醇来源于 SBHH。总的来说，将细胞固定在木质纤维素材料上可以成为一种有效的策略，从重复分批发酵未经解毒的半纤维素水解物中生产丁醇。
