School of Materials Science and Engineering, Zhengzhou University, Zhengzhou, China.
School of Materials Science and Engineering, Zhengzhou University, Zhengzhou, China.
Int J Biol Macromol. 2021 Jan 31;168:233-241. doi: 10.1016/j.ijbiomac.2020.12.056. Epub 2020 Dec 9.
Herein, oxidized konjac glucomannan (OKG) with different molecular weight (M) were prepared as polysaccharide crosslinker to reinforce gelatin-based hydrogels. Then, properties of composite hydrogels with various OKGs were investigated via a series of methods, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), rheology, mechanical and biocompatibility tests. The results confirmed an increased degree of crosslinking and entanglement between gelatin and OKG with higher M. Besides, composite hydrogels not only showed increased mechanical strength, but self-healing ability at the same time, which were closely affected by the M of OKG. Furthermore, both composite hydrogels could support well proliferation of cells, which showed excellent capacity in tissue engineering and biomedical applications. In brief, this work provides a facile method to promote the overall properties of gelatin-based hydrogels, meanwhile revealed the relationship and mechanism underlying the effects of OKG with different M on composite hydrogels.
在此，我们制备了具有不同分子量（M）的氧化魔芋葡甘聚糖（OKG）作为多糖交联剂来增强明胶基水凝胶。然后，通过一系列方法研究了具有不同 OKG 的复合水凝胶的性能，包括傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、流变学、机械性能和生物相容性测试。结果证实，随着 M 的增加，明胶与 OKG 之间的交联程度和缠结程度增加。此外，复合水凝胶不仅表现出更高的机械强度，同时还具有自修复能力，这与 OKG 的 M 密切相关。此外，两种复合水凝胶均能很好地促进细胞的增殖，在组织工程和生物医学应用方面具有优异的性能。总之，本工作提供了一种促进明胶基水凝胶整体性能的简便方法，同时揭示了不同 M 的 OKG 对复合水凝胶影响的关系和机制。
