School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Int J Biol Macromol. 2016 Oct;91:358-67. doi: 10.1016/j.ijbiomac.2016.05.042. Epub 2016 May 12.
Polysaccharide hydrogels have been widely used as biomaterials in biomedical field. In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS). Meanwhile, different amount of graphene oxide (GO) was added as nano-additive. The hydrogels have been characterized by various methods including Fourier transform infrared spectroscopy (FT-IR) and Surface morphology (SEM). Through the observation of SEM, the hydrogels' scaffolds present a homogeneous interconnected pore structure after lyophilizing. In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated. The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate. Especially, the compressive strength and modulus increase 144% and 296% respectively as the GO content increase from 0 to 5mg/ml. Moreover, MTT assay was applied to evaluate the biocompatibility of hydrogels. The result indicate that hydrogels with GO show better biocompatibility. Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
多糖水凝胶作为生物材料已广泛应用于生物医学领域。本文通过氧化魔芋葡甘聚糖（OKGM）的醛基与羧甲基壳聚糖（CMCS）的氨基之间的席夫碱反应制备了复合水凝胶。同时，添加了不同量的氧化石墨烯（GO）作为纳米添加剂。通过傅里叶变换红外光谱（FT-IR）和表面形貌（SEM）等多种方法对水凝胶进行了表征。通过 SEM 的观察，冻干后的水凝胶支架呈现出均匀的互连孔结构。此外，研究了不同 GO 含量对凝胶时间、溶胀能力、水蒸发率和机械性能等性能的影响。结果表明，水凝胶具有较短的凝胶时间、适当的溶胀能力和水蒸发率。特别是，随着 GO 含量从 0 增加到 5mg/ml，压缩强度和模量分别增加了 144%和 296%。此外，还通过 MTT 试验评价了水凝胶的生物相容性。结果表明，含有 GO 的水凝胶具有更好的生物相容性。因此，由于具有适当的吸水能力、与软组织相似的压缩模量和优异的生物相容性，复合水凝胶在伤口敷料中有潜在的应用前景。
