Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457, PR China; Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Biol Macromol. 2021 Feb 1;169:18-27. doi: 10.1016/j.ijbiomac.2020.12.033. Epub 2020 Dec 9.
Fumonisins have posed hazardous threat to human and animal health worldwide. Enzymatic degradation is a desirable detoxification approach but is severely hindered by serious shortage of detoxification enzymes. After mining enzymes by bioinformatics analysis, a novel carboxylesterase FumDSB from Sphingomonadales bacterium was expressed in Escherichia coli, and confirmed to catalyze fumonisin B1 to produce hydrolyzed fumonisin B1 by liquid chromatography mass spectrometry for the first time. FumDSB showed high sequence novelty, sharing only ~34% sequence identity with three reported fumonisin detoxification carboxylesterases. Besides, FumDSB displayed its high degrading activity at 30-40 °C within a broad pH range from 6.0 to 9.0, which is perfectly suitable to be used in animal physiological condition. It also exhibited excellent pH stability and moderate thermostability. This study provides a FB1 detoxification carboxylesterase which could be further used as a potential food and feed additive.
伏马菌素对全球人类和动物健康构成了严重威胁。酶法降解是一种理想的解毒方法，但严重缺乏解毒酶。通过生物信息学分析挖掘酶后，首次在大肠杆菌中表达了来自鞘氨醇单胞菌的新型羧酸酯酶 FumDSB，并通过液相色谱-质谱联用技术证实其能催化伏马菌素 B1 生成水解伏马菌素 B1。FumDSB 具有很高的序列新颖性，与已报道的三种伏马菌素解毒羧酸酯酶的序列相似性仅约为 34%。此外，FumDSB 在 30-40°C 的较宽温度范围内和 pH6.0-9.0 的较宽 pH 范围内均表现出很高的降解活性，非常适合在动物生理条件下使用。它还表现出良好的 pH 稳定性和中等的热稳定性。本研究提供了一种 FB1 解毒羧酸酯酶，可进一步作为一种有潜力的食品和饲料添加剂。
