Oka Y, Fujiwara K, Endou H
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Ren Physiol. 1987;10(5):283-8. doi: 10.1159/000173136.
Using a specific and sensitive epidermal growth factor (EGF) radioimmunoassay, the distribution of EGF in mouse kidneys was studied. In five portions (outer cortex, inner cortex, outer medulla I, II, and papilla) of kidney slices, the outer medulla revealed the highest immunoreactive EGF (iEGF) per wet weight of tissue. In microdissected nephron segments, the predominant iEGF per millimeter length was observed in the medullary and cortical thick ascending limbs of Henle's loop compared with other segments. It became more apparent when expressed per milligram protein. Fluorescence microscopy showed prominent staining for EGF in the thick ascending limbs of Henle's loop. The significance of the nephron heterogeneity in EGF contents is discussed.
采用特异性强且灵敏的表皮生长因子（EGF）放射免疫分析法，对小鼠肾脏中EGF的分布进行了研究。在肾脏切片的五个部分（外皮质、内皮质、外髓质I、II和乳头）中，外髓质每单位组织湿重的免疫反应性EGF（iEGF）最高。在显微解剖的肾单位节段中，与其他节段相比，在髓袢的髓质和皮质厚升支中观察到每毫米长度的主要iEGF。以每毫克蛋白质表示时，这一点变得更加明显。荧光显微镜显示髓袢厚升支中有明显的EGF染色。讨论了肾单位在EGF含量方面的异质性的意义。
