State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No.38 Xueyuan Road, Beijing 100191, China.
Center for Drug Evaluation, China Food and Drug Administration, No.1 Fuxing Road, Beijing 100038, China.
J Agric Food Chem. 2020 Dec 23;68(51):15164-15175. doi: 10.1021/acs.jafc.0c04247. Epub 2020 Dec 14.
Ononin is a bioactive isoflavone of legumes. To explore the "effective forms" of ononin, its metabolites were characterized using HPLC-ESI-IT-TOF-MS after oral administration to rats. Metabolites (106), including 94 new metabolites, were characterized, which contained 17 phase I, 23 hydroxylated and methylated, 54 sulfated, 10 glucuronidated, and 2 sulfated and glucuronidated metabolites. Six hydroxylated metabolites of formononetin (aglycone of ononin) were simultaneously detected for the first time. Twenty-three hydroxylated and methylated metabolites were the new metabolites of ononin, and the number of hydroxylation and methylation was 1-3 and 1-2. Twenty metabolites have ononin-related bioactivities, and many metabolites have the same bioactivities. Their probable mechanisms of action may be additive and/or synergistic effects, especially because of the addition of the blood concentrations of these compounds. The results provide a foundation for a better understanding of the "effective forms" of ononin.
染料木黄酮是豆科植物中的一种生物活性异黄酮。为了探索染料木黄酮的“有效形式”，我们采用 HPLC-ESI-IT-TOF-MS 法在大鼠口服给药后对其代谢产物进行了表征。共鉴定出 106 种代谢产物，包括 94 种新代谢产物，其中包含 17 种Ⅰ相代谢产物、23 种羟化和甲基化产物、54 种硫酸化产物、10 种葡萄糖醛酸化产物和 2 种硫酸化和葡萄糖醛酸化产物。首次同时检测到黄豆黄素（染料木黄酮的苷元）的 6 种羟化代谢产物。23 种羟化和甲基化代谢产物是染料木黄酮的新代谢产物，羟化和甲基化的数量为 1-3 和 1-2。20 种代谢产物具有与染料木黄酮相关的生物活性，许多代谢产物具有相同的生物活性。它们的可能作用机制可能是相加和/或协同作用，特别是由于这些化合物的血液浓度增加。研究结果为更好地理解染料木黄酮的“有效形式”提供了依据。
