Venier P, Tecchio G, Clonfero E, Levis A G
Department of Biology, University of Padua, Italy.
Mutagenesis. 1987 Jan;2(1):19-22. doi: 10.1093/mutage/2.1.19.
Seven carbon black pastes used as commercial leather dyes were tested for their mutagenicity in the Salmonella/microsome test (TA98 and TA100 strains). All the samples assayed either directly or after extraction with a 30-min sonication in benzene were devoid of mutagenicity both in the presence and absence of a metabolic activation preparation. After a 48-h extraction with boiling toluene in a Soxhlet apparatus, four samples were mutagenic in TA98 strain in the presence of S9 mix. The activity ranged from 1.3 to 9.6 induced revertants/mg equivalent of extract. A weak direct mutagenic activity in strain TA98 was shown by one extract. Polycyclic aromatic hydrocarbons (PAH) were determined in the toluene extracts by high resolution gas chromatography/mass spectrometry. The presence of PAH could explain the mutagenicity of only one sample (8.79 micrograms of total PAH/100 mg equivalents of extract), while low or undetectable levels of PAH were found in the other mutagenic extracts. The mutagenic activity was evident only after a vigorous extraction process, thus a low bioavailability of the mutagens present in these compounds is suggested.
对七种用作商业皮革染料的炭黑糊剂进行了沙门氏菌/微粒体试验（TA98和TA100菌株）中的致突变性测试。所有直接检测的样品或在苯中超声处理30分钟后萃取的样品，无论有无代谢活化制剂存在，均无致突变性。在索氏提取器中用沸腾甲苯萃取48小时后，四个样品在S9混合物存在下对TA98菌株具有致突变性。活性范围为每毫克提取物当量1.3至9.6个诱导回复突变体。一种提取物在TA98菌株中显示出较弱的直接致突变活性。通过高分辨率气相色谱/质谱法测定甲苯提取物中的多环芳烃（PAH）。PAH的存在仅能解释一个样品的致突变性（每100毫克提取物当量含8.79微克总PAH），而在其他致突变提取物中发现PAH含量较低或无法检测到。致突变活性仅在剧烈萃取过程后才明显，因此表明这些化合物中存在的诱变剂生物利用度较低。
