Noda M, Ikawa Y
Laboratory of Molecular Oncology, Riken Tsukuba Life Science Center, Ibaraki, Japan.
Princess Takamatsu Symp. 1986;17:261-7.
We have tried to detect genes which are expressed in normal cells and have the potential of suppressing transforming activity of the Kirsten sarcoma virus oncogene, v-Ki-ras. The experiments involved isolation and characterization of flat revertants from v-Ki-ras-transformed NIH/3T3 cells following either chemical mutagenesis or transfection of a cDNA expression library constructed from total mRNA of normal human fibroblasts. By these procedures multiple genes could be detected which, when transcriptionally activated, suppress different spectra of transformation-associated properties in spite of persistent expression of the v-Ki-ras gene product.
我们试图检测在正常细胞中表达且具有抑制柯斯顿肉瘤病毒癌基因v-Ki-ras转化活性潜力的基因。实验包括从经化学诱变或转染由正常人成纤维细胞总mRNA构建的cDNA表达文库后的v-Ki-ras转化的NIH/3T3细胞中分离并鉴定扁平回复突变体。通过这些方法，可以检测到多个基因，这些基因在转录激活后，尽管v-Ki-ras基因产物持续表达，但仍能抑制不同谱的转化相关特性。
