Ogawa Yuko, Okinaka Yuka, Takeuchi Yukiko, Saino Orie, Kikuchi-Taura Akie, Taguchi Akihiko
Department of Regenerative Medicine Research, Foundation for Biomedical Research and Innovation at Kobe, Hyogo, Japan.
Front Med (Lausanne). 2020 Nov 26;7:535902. doi: 10.3389/fmed.2020.535902. eCollection 2020.
There is no effective treatment for chronic stroke if the acute or subacute phase is missed. Rehabilitation alone cannot easily achieve a dramatic recovery in function. In contrast to significant therapeutic effects of bone marrow mononuclear cells (BM-MNC) transplantation for acute stroke, mild and non-significant effects have been shown for chronic stroke. In this study, we have evaluated the effect of a combination of BM-MNC transplantation and neurological function training in chronic stroke. The effect of BM-MNC on neurological functional was tested four weeks after permanent middle cerebral artery occlusion (MCAO) insult in mice. BM-MNC (1 × 10cells in 100 μl PBS) were injected into the vein of MCAO model mice, followed by behavioral tests as functional evaluations. Interestingly, there was a significant therapeutic effect of BM-MNC only when repeated training was performed. This suggested that cell therapy alone was not sufficient for chronic stroke treatment; however, training with cell therapy was effective. The combination of these differently targeted therapies provided a significant benefit in the chronic stroke mouse model. Therefore, targeted cell therapy BM-MNC transplantation with appropriate training presents a promising novel therapeutic option for patients in the chronic stroke period.
如果错过急性或亚急性期，慢性中风就没有有效的治疗方法。仅靠康复难以轻易实现功能的显著恢复。与骨髓单个核细胞（BM-MNC）移植对急性中风的显著治疗效果相比，其对慢性中风的效果轻微且不显著。在本研究中，我们评估了BM-MNC移植与神经功能训练相结合对慢性中风的影响。在小鼠永久性大脑中动脉闭塞（MCAO）损伤四周后，测试BM-MNC对神经功能的影响。将BM-MNC（1×10个细胞溶于100μl PBS中）注入MCAO模型小鼠的静脉，随后进行行为测试作为功能评估。有趣的是，只有在进行重复训练时，BM-MNC才具有显著的治疗效果。这表明仅细胞治疗不足以治疗慢性中风；然而，细胞治疗与训练相结合是有效的。这些不同靶向治疗方法的组合在慢性中风小鼠模型中带来了显著益处。因此，靶向细胞治疗——BM-MNC移植与适当训练相结合，为处于慢性中风期的患者提供了一种有前景的新型治疗选择。
