Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, GSP-1, Leninskie gory 1, build. 3, Moscow 119991, Russia.
Polymer Department, Faculty of Chemistry, Lomonosov Moscow State University, GSP-1, Leninskie gory 1, build. 3, Moscow 119991, Russia.
Biomacromolecules. 2021 Feb 8;22(2):681-689. doi: 10.1021/acs.biomac.0c01504. Epub 2020 Dec 16.
We report here the first evidence for the interaction of poly(ethylene glycol) (PEG) with hyaluronan (HA) in aqueous solutions. PEG-HA complexes ( = 45,000 ± 8000 M) contained about 3.3 ± 0.1 of ethylene glycol units per disaccharide of HA. The carboxyl of the D-glucuronic acid and the amide of the N-acetyl-D-glucosamine did not participate in PEG binding. Similar experiments performed with dextran and monosaccharides showed that multiple free primary hydroxyls regularly distributed along the polysaccharide chain are necessary for PEG binding. Another novelty of our study is contraction of HA upon PEG binding. The effect was observed with HA in solution or adsorbed on positively charged liposomes. The thickness of the HA layer on the liposomes decreased 2-fold upon PEG addition. HA compaction induced by PEG may underlie the changes in the plasma membrane properties and resealing of mechanical injuries induced by Pluronics.
我们在这里报告了聚乙二醇（PEG）与透明质酸（HA）在水溶液中相互作用的第一个证据。PEG-HA 复合物（=45000±8000M）中每一个 HA 的二糖单元大约含有 3.3±0.1 个乙二醇单元。D-葡萄糖醛酸的羧基和 N-乙酰-D-葡萄糖胺的酰胺没有参与 PEG 的结合。用葡聚糖和单糖进行的类似实验表明，多糖链上均匀分布的多个游离伯羟基是 PEG 结合所必需的。我们研究的另一个新发现是 PEG 结合导致 HA 收缩。这种效应在溶液中的 HA 或吸附在带正电荷的脂质体上都观察到了。加入 PEG 后，脂质体上 HA 层的厚度减少了 2 倍。PEG 诱导的 HA 紧缩可能是导致 Pluronic 诱导的质膜性质变化和机械损伤修复的原因。
