Department of Kinesiology, School of Public Health, Indiana University, Bloomington, Indiana, USA.
The Center for RNA Science and Therapeutics, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
High Alt Med Biol. 2021 Jun;22(2):148-156. doi: 10.1089/ham.2020.0190. Epub 2020 Dec 15.
Baranauskas, Marissa N., Joseph Powell, Alyce D. Fly, Bruce J. Martin, Timothy D. Mickleborough, Hunter L. Paris, and Robert F. Chapman. Influence of zinc on the acute changes in erythropoietin and proinflammatory cytokines with hypoxia. . 22: 148-156, 2021. Considerable, unexplained, interindividual variability characterizes the erythropoietin (EPO) response to hypoxia, which can impact hematological acclimatization for individuals sojourning to altitude. Zinc supplementation has the potential to alter EPO by attenuating increases in inflammation and oxidative stress. Yet, the application of such an intervention has not been evaluated in humans. In this proof-of-concept study, we aimed to evaluate the EPO and inflammatory responses to acute hypoxia in human participants following chronic zinc supplementation. Nine physically active participants (men = 5, women = 4, age 28 ± 4 years, height 176 ± 11 cm, mass 77 ± 21 kg) were exposed to 12 hours of normobaric hypoxia simulating an altitude of 3,000 m (FO = 0.14) before and after 8 weeks of supplementation with 40 mg/day of elemental zinc from picolinate. Blood samples for subsequent analysis of serum zinc, EPO, superoxide dismutase (extracellular superoxide dismutase [EC-SOD]), C-reactive protein (CRP), and proinflammatory cytokines were obtained pre- and postsupplementation and exposure to hypoxia. After zinc supplementation, EPO increased by 64.9 ± 36.0% (mean ± standard deviation) following 12 hours of hypoxia, but this response was not different from presupplementation (70.8 ± 46.1%). Considerable interindividual (range: -1% to +208%) variability was apparent in the acute EPO response. While most markers of inflammation did not change with hypoxia, interleukin-6 concentrations increased from 1.17 ± 0.05 to 1.97 ± 0.32 pg/ml during the final 6 hours. The acute EPO response at 12 hours was not related to changes in serum zinc, EC-SOD, CRP, or proinflammatory cytokines. Zinc supplementation does not influence the acute EPO or inflammatory response with short-term exposure to moderate levels of normobaric hypoxia (3,000 m) in apparently healthy young adults.
巴兰努斯卡丝，玛丽莎 N.，约瑟夫·鲍威尔，艾丽斯·D. 弗莱，布鲁斯 J. 马丁，蒂莫西·D. 米克尔伯勒，亨特 L. 巴黎，罗伯特 F. 查普曼。锌对急性缺氧时促红细胞生成素和促炎细胞因子的影响。. 22: 148-156, 2021. 大量的、未被解释的个体间变异性是促红细胞生成素（EPO）对缺氧反应的特征，这可能会影响个体在海拔地区的血液适应。锌补充剂有可能通过减轻炎症和氧化应激的增加来改变 EPO。然而，这种干预措施的应用尚未在人类中得到评估。在这项概念验证研究中，我们旨在评估慢性锌补充后，人类参与者在急性缺氧下的 EPO 和炎症反应。 9 名身体活跃的参与者（男性 5 名，女性 4 名，年龄 28±4 岁，身高 176±11cm，体重 77±21kg）在接受 12 小时的常压缺氧模拟海拔 3000 米（FO=0.14）之前和之后接受了 8 周的补充，每天补充 40mg 的锌来自吡啶酸。在补充和暴露于缺氧之前，采集血液样本，用于后续分析血清锌、EPO、超氧化物歧化酶（细胞外超氧化物歧化酶 [EC-SOD]）、C 反应蛋白（CRP）和促炎细胞因子。 锌补充后，12 小时的缺氧后 EPO 增加了 64.9±36.0%（平均值±标准差），但与补充前相比没有差异（70.8±46.1%）。急性 EPO 反应的个体间（范围：-1%至+208%）存在明显的变异性。虽然大多数炎症标志物在缺氧时没有变化，但白细胞介素-6 浓度在最后 6 小时内从 1.17±0.05 增加到 1.97±0.32pg/ml。在 12 小时的急性 EPO 反应与血清锌、EC-SOD、CRP 或促炎细胞因子的变化无关。 在健康年轻成年人中，短期暴露于中海拔水平（3000 米）的常压缺氧下，锌补充剂不会影响急性 EPO 或炎症反应。
