Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Peru.
Escuela Profesional de Medicina Humana, Universidad Privada San Juan Bautista, Lima, Peru.
Sci Rep. 2020 Dec 16;10(1):22080. doi: 10.1038/s41598-020-78808-y.
Salmonella Enteritidis, an important foodborne zoonosis, has a dramatically increased number of cases around the world. To explore the phylogenetic structure of Peruvian Salmonella Enteritidis strains and their relationship with an outbreak occurred in 2018, we analyzed a comprehensive strains of S. Enteritidis received by the National Institute of Health during the period 2000-2018. A total of 180 strains were characterized by microbiological procedures, serotyping and whole genome sequencing. Based on genome sequences annotated, virulence factors and accessory genes were identified. Phylogenetic and population structure analysis were also analyzed based on SNPs. The phylogenetic analysis grouped the genomes into two well-supported clades that were consistent with population structure analysis. The clinical and food strains corresponding to the outbreak were included in the same cluster, which presented the sdhA gene, related to the increase of the virulence of this pathogen. The phylogenetic relationship of Peruvian S. Enteritidis suggests the presence of four S. enteritidis population with high epidemiological importance.
肠炎沙门氏菌是一种重要的食源性人畜共患病，在世界各地的病例数量急剧增加。为了探索秘鲁肠炎沙门氏菌菌株的系统发育结构及其与 2018 年爆发疫情的关系，我们对秘鲁国家卫生研究所 2000-2018 年期间收到的综合肠炎沙门氏菌菌株进行了分析。对 180 株菌株进行了微生物学程序、血清型和全基因组测序的特征分析。根据基因组序列注释，确定了毒力因子和辅助基因。还基于 SNPs 进行了系统发育和群体结构分析。系统发育分析将基因组分为两个得到很好支持的分支，与群体结构分析一致。与疫情相关的临床和食品菌株包含在同一簇中，该簇存在与该病原体毒力增加相关的 sdhA 基因。秘鲁肠炎沙门氏菌的系统发育关系表明，存在四个具有高度流行病学重要性的肠炎沙门氏菌种群。
