Liu T F, Lin T, Ren L H, Li G P, Peng J J
Beijing Da Xue Xue Bao Yi Xue Ban. 2020 Dec 18;52(6):1082-1087. doi: 10.19723/j.issn.1671-167X.2020.06.015.
To elucidate the correlation between CKLF-like MARVEL transmembrane domain containing member 5 () gene and the risk of coronary artery disease (CAD), and to detect the effects of gene expression changes on the ability of adhesion and migration of THP-1 cells.
Using case-control method, a total of 700 hospitalized patients in Shijitan Hospital were enrolled in this study. CAD were diagnosed by coronary angiography, which was defined as at least one blood vessel diameter stenosis ≥50% according to the result of coronary angiography. Reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect gene expression; enzyme linked immunosorbent assay (ELISA) method to detect the plasma level of CMTM5; and Logistic regression to analyze genes and the risk of CAD. Human vascular endothelial cells (ECs) and THP-1 cells were cultivated, adhesion and Transwells experiments were used to evaluate the chemotactic capabi-lity of gene on THP-1 cells.
In this study, 350 CAD patients matched with 350 control patients were included. RT-PCR results revealed mRNA expression in CAD group was 3.45 times compared with control group, which was significantly higher than that in control group ( < 0.05). The levels of CMTM5 plasma protein in CAD group was (206.1±26.9) μg/L, which was significantly higher than that in control group (125.3±15.2) μg/L ( < 0.05). After adjusted for the risk factors of age, gender, BMI, smoking, hypertension, diabetes and hyperlipidemia, Logistic regression analysis results indicated that was the susceptibility factors of CAD, which still had significant correlation with CAD ( < 0.05). Adhesion and Transwells experiments results revealed that the numbers of adhesion and migration of THP-1 cells in overexpression ECs group (EO group) were significantly higher than that in lenti-mock infected ECs group (EO-MOCK group), non-infected ECs group (EN group), lenti-mock infected ECs group (ES-MOCK group), and suppression ECs group (ES group). On the contrary, the numbers of adhesion and migration of THP-1 cells in ES group were significantly lower than that in the other four groups ( < 0.01).
gene was closely related to the development of CAD. overexpression promoted the adhesion and migration of THP-1, which might play a part in the mechanisms of atherosclerosis and CAD.
阐明含CKLF样MARVEL跨膜结构域成员5（CMTM5）基因与冠状动脉疾病（CAD）风险之间的相关性，并检测CMTM5基因表达变化对THP-1细胞黏附及迁移能力的影响。
采用病例对照研究方法，选取北京世纪坛医院住院患者700例。通过冠状动脉造影诊断CAD，根据冠状动脉造影结果，将至少一支血管直径狭窄≥50%定义为CAD。采用逆转录-聚合酶链反应（RT-PCR）法检测CMTM5基因表达；采用酶联免疫吸附测定（ELISA）法检测血浆CMTM5水平；采用Logistic回归分析CMTM5基因与CAD风险的关系。培养人血管内皮细胞（ECs）和THP-1细胞，采用黏附实验及Transwell实验评估CMTM5基因对THP-1细胞的趋化能力。
本研究共纳入350例CAD患者和350例对照患者。RT-PCR结果显示，CAD组CMTM5 mRNA表达量是对照组的3.45倍，显著高于对照组（P<0.05）。CAD组血浆CMTM5蛋白水平为（206.1±26.9）μg/L，显著高于对照组（125.3±15.2）μg/L（P<0.05）。在调整年龄、性别、BMI、吸烟、高血压、糖尿病和高脂血症等危险因素后，Logistic回归分析结果显示，CMTM5是CAD的易感因素，与CAD仍具有显著相关性（P<0.05）。黏附实验及Transwell实验结果显示，CMTM5过表达ECs组（EO组）中THP-1细胞的黏附及迁移数量显著高于慢病毒空载感染ECs组（EO-MOCK组）、未感染ECs组（EN组）、慢病毒空载感染ECs组（ES-MOCK组）及CMTM5抑制ECs组（ES组）。相反，ES组中THP-1细胞的黏附及迁移数量显著低于其他四组（P<0.01）。
CMTM5基因与CAD的发生发展密切相关。CMTM5过表达促进THP-1细胞的黏附及迁移，可能参与动脉粥样硬化及CAD的发病机制。
