一种改良的兔气管狭窄模型及家用内窥镜。更简单且更便于使用。
A modified rabbit model of tracheal stenosis and a household endoscope. More simplicity and accessibility.
作者信息
Zhang Guoying, Wang Jianming, Zeng Yiming
机构信息
PhD, Department of Pulmonary and Critical Care Medicine, Respiratory Medicine Center of Fujian Province, The Second Affiliated Hospital of Fujian Medical University, and Department of Emergency Medicine, Quanzhou First Hospital of Fujian Medical University, Quanzhou, Fujian, China. Substantive scientific and intellectual contributions to the study; conception and design; acquisition, analysis and interpretation of data; histopathological examinations; statistics analysis; manuscript preparation and writing; final approval.
Master, Department of Pulmonary and Critical Care Medicine, Respiratory Medicine Center of Fujian Province, The Second Affiliated Hospital of Fujian Medical University, and Department of Emergency Medicine, Quanzhou First Hospital of Fujian Medical University, Quanzhou, Fujian, China. Substantive scientific and intellectual contributions to the study, manuscript preparation and writing, critical revision, final approval.
出版信息
Acta Cir Bras. 2020 Dec 18;35(11):e351104. doi: 10.1590/ACB351104. eCollection 2020.
PURPOSE
To develop a simpler animal model for benign tracheal stenosis and introduce a low-cost household endoscope for postmodeling endotracheal evaluation.
METHODS
Twenty rabbits were randomly divided into a model group (15 rabbits, subjected to transoral nylon brush scraping of the trachea) and a mock group (5 rabbits, merely exempted from scraping), a household endoscope was then introduced for weekly endoscopic examination. Meanwhile, other 15 rabbits (modeling like the model group) underwent batch tracheal resection at different postintervention times for pathological analysis.
RESULTS
The model group presented a low mortality and few complications. The endoscope could obtain adequate images for stenosis assessment, which showed that the models presented homogeneous injury after scraping and developed a mature scar stricture at 28 days postoperatively with a mean stenosis degree of 65.9%, and 71.4% (10/14) above Myer-Cotton's grade II. The pathological findings were consistent with the clinicopathological process of human. No stenosis was found in mock group.
CONCLUSION
The modified model is simpler, minimally invasive and reliable, while the household endoscope is competent for model's follow-up, providing easily accessible and useful tools for facilitating more extensive studies of benign tracheal stenosis.
目的
建立一种更简单的良性气管狭窄动物模型，并引入一种低成本家用内窥镜用于建模后气管内评估。
方法
将20只兔子随机分为模型组（15只兔子，经口行气管尼龙刷刮擦）和假手术组（5只兔子，仅不行刮擦），然后引入家用内窥镜进行每周一次的内镜检查。同时，另外15只兔子（建模方式同模型组）在干预后的不同时间分批进行气管切除以进行病理分析。
结果
模型组死亡率低且并发症少。内窥镜能够获取足够的图像用于狭窄评估，结果显示模型在刮擦后呈现均匀损伤，术后28天形成成熟的瘢痕狭窄，平均狭窄程度为65.9%，71.4%（10/14）高于迈耶 - 科顿二级。病理结果与人类临床病理过程一致。假手术组未发现狭窄。
结论
改良后的模型更简单、微创且可靠，而家用内窥镜能够胜任模型的随访工作，为促进更广泛的良性气管狭窄研究提供了易于获取且有用的工具。
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