Wang Y N, Wyatt R J
Neuropsychiatry Branch, National Institute of Mental Health, Saint Elizabeths Hospital, Washington, DC 20032.
Synapse. 1987;1(2):208-13. doi: 10.1002/syn.890010209.
The immunohistochemical localization of peptide F and the related enkephalins met5-enkephalin (met-enk), leu5-enkephalin (leu-enk), met5-enkephalin-arg6-phe7 (met-enk-arg-phe), and met5-enkephalin-arg6-gly7-leu8 (met-enk-arg-gly-leu) was investigated by means of the indirect immunofluorescence technique in the enteric nervous system of the rat. Peptide F-like immunoreactivity was widely distributed within neuronal structures throughout the gastrointestinal (GI) tract of the rat. Peptide F-containing nerve cell bodies were mainly located in the myenteric plexus, and only rarely were seen in the submucosal plexus. Peptide F-immunoreactive nerve fibers were principally present in the myenteric plexus and circular muscle layer; few were found in the submucosal plexus, longitudinal muscle layer, muscularis mucosa, and mucosa. No peptide F-containing fibers were found associated with blood vessels in the gut. By comparing the distribution of immunoreactive peptide F with other related enkephalins met-enk, leu-enk, met-enk-arg-phe, and met-enk-arg-gly-leu, we observed that there was a remarkable similarity in the distribution of peptide F and other enkephalins in the GI tract. These data, combined with our previous studies, indicate that peptide F may coexist with other related enkephalins in the same neurons of the enteric nervous system. The results suggest that peptide F, a product of the proenkephalin A gene, may play a physiological role within the enteric nervous system.
采用间接免疫荧光技术，对大鼠肠神经系统中肽F以及相关脑啡肽（甲硫氨酸脑啡肽（met-enk）、亮氨酸脑啡肽（leu-enk）、甲硫氨酸脑啡肽-精氨酸-苯丙氨酸（met-enk-arg-phe）和甲硫氨酸脑啡肽-精氨酸-甘氨酸-亮氨酸（met-enk-arg-gly-leu））进行免疫组织化学定位研究。肽F样免疫反应性广泛分布于大鼠整个胃肠道的神经元结构内。含肽F的神经细胞体主要位于肌间神经丛，在黏膜下神经丛中很少见到。肽F免疫反应性神经纤维主要存在于肌间神经丛和环肌层；在黏膜下神经丛、纵肌层、黏膜肌层和黏膜中发现较少。在肠道中未发现与血管相关的含肽F纤维。通过比较免疫反应性肽F与其他相关脑啡肽（met-enk、leu-enk、met-enk-arg-phe和met-enk-arg-gly-leu）的分布，我们观察到肽F与其他脑啡肽在胃肠道中的分布有显著相似性。这些数据与我们之前的研究相结合，表明肽F可能与其他相关脑啡肽在肠神经系统的同一神经元中共存。结果表明，作为前脑啡肽A基因产物的肽F可能在肠神经系统中发挥生理作用。
