Department of General Practice, Sir Run Run Shaw Hospital, Zhejiang University, China.
Department of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, China.
Life Sci. 2021 Feb 1;266:118909. doi: 10.1016/j.lfs.2020.118909. Epub 2020 Dec 15.
To investigate the mechanism of Ghrelin/GHS-R signaling pathway in small intestine injury induced by NSAIDs related enteropathy. To clarify the mechanism network of intestinal mucosal repair with naringin as a new therapeutic method.
Naringin was used as the intervention method, observed the damage of small intestinal mucosa and detected the expression of ghrelin, GHS-R, leptin and TNF-α by electron microscopy, HE staining and immunohistochemistry.
Compared with the control group, the weight of rats in the model group decreased, the thickness of intestinal mucosa became thinner, the structure of intestinal mucosa changed, the expression of ghrelin, GHS-R and leptin decreased, the expression of TNF-α increased. Compared with the model group, the intestinal mucosa of the treatment group was repaired, the expression of ghrelin, GHS-R and leptin was increased, and the expression TNF-α was decreased.
The mechanism of intestinal mucosal damage in patients with NSAIDs related enteropathy may be related to the decreased expression of ghrelin, GHS-R and leptin, and promotion of TNF-α secretion. Naringin can effectively promote the secretion of ghrelin and leptin, the expression of GSH-R, and inhibit the release of TNF-α, so as to repair intestinal mucosa naringin will become a new method to treat and prevent NSAIDs related intestinal diseases.
研究 Ghrelin/GHS-R 信号通路在 NSAIDs 相关肠病引起的小肠损伤中的作用机制，阐明柚皮苷作为一种新的治疗方法促进肠黏膜修复的机制网络。
以柚皮苷为干预方法，通过电镜、HE 染色和免疫组织化学观察小肠黏膜的损伤情况，检测 ghrelin、GHS-R、瘦素和 TNF-α的表达。
与对照组相比，模型组大鼠体重减轻，肠黏膜变薄，肠黏膜结构发生改变，ghrelin、GHS-R 和瘦素表达减少，TNF-α表达增加。与模型组相比，治疗组的肠黏膜得到修复，ghrelin、GHS-R 和瘦素的表达增加，TNF-α的表达减少。
NSAIDs 相关肠病患者肠黏膜损伤的机制可能与 ghrelin、GHS-R 和瘦素表达减少，促进 TNF-α 分泌有关。柚皮苷能有效促进 ghrelin 和瘦素的分泌，GSH-R 的表达，抑制 TNF-α 的释放，从而修复肠黏膜，柚皮苷将成为治疗和预防 NSAIDs 相关肠道疾病的新方法。
