Forcibly spun dragline silk fibers from web-building spider Trichonephila clavata ensure robustness irrespective of spinning speed and humidity.

Web-building spiders secrete dragline silk fibers to sustain their body and use them as frameworks during web construction. They spin dragline silk fibers at various spinning speed and humidity conditions depending on their natural habitat. Here, we investigated the effect of spinning speed and humidity on the structural and mechanical properties of dragline silk fibers from web-building spider Trichonephila clavata obtained by the forcibly spinning method. We found that the crystal and morphological structures did not rely on the spinning speed and humidity. Furthermore, the mechanical strength and extensibility of the dragline silk fibers were maintained, demonstrating that dragline silk fibers ensure robustness irrespective of the spinning speed and humidity. The results obtained in the present study are helpful not only to understand the biological basis of the silk fiber formation of spiders but also contribute to consider the spinning conditions for the process of creating synthetic silk fibers.