Wang Guo-Hua, Wang Liang-Yan, Zhang Cui, Zhang Peng, Wang Chuan-Hui, Cheng Shuai
Department of Neurosurgery, Sunshine Union Hospital of Shandong Province, No. 9000 Yingqian Street, Weifang, Shandong, 261000, People's Republic of China.
Department of Ophthalmology, The Affiliated Hospital of Weifang Medical University, Weifang, Shandong , 261000, People's Republic of China.
Open Med (Wars). 2020 Sep 9;15(1):872-881. doi: 10.1515/med-2020-0156. eCollection 2020.
This study attempted to research the molecular mechanism underlying the inhibitory role of miR-1225-5p in the malignant progression of glioblastoma. Bioinformatics analyses based on the gene expression omnibus (GEO) and Chinese glioma genome atlas (CGGA) databases showed that miR-1225-5p, as a favorable prognostic factor, was expressed at low levels in glioblastoma, and its expression was also related to WHO grade and age. The subsequent CCK-8 assay indicated that miR-1225-5p might prevent the malignant progression of glioblastoma, which was represented by that miR-1225-5p mimic reduced the viability of glioblastoma cells. Then, we predicted that might be a potential target gene of miR-1225-5p, and it was negatively correlated with the level of miR-1225-5p, which were confirmed by dual-luciferase reporter assay, qRT-PCR and western blot assays. Moreover, based on the analyses of the cancer genome atlas (TCGA), Oncomine and CGGA databases, was enriched in glioblastoma and high expression of led to poor prognosis. Finally, CCK8 and transwell experiments showed that the ability of miR-1225-5p to inhibit glioblastoma cell viability, invasion and migration was at least partially achieved by targeting . In general, these results revealed that the miR-1225-5p/ axis contributes to inhibiting the malignant phenotype of glioblastoma cells, which lays a foundation for molecular diagnosis and treatment of glioblastoma.
本研究试图探究miR-1225-5p在胶质母细胞瘤恶性进展中发挥抑制作用的分子机制。基于基因表达综合数据库（GEO）和中国胶质瘤基因组图谱（CGGA）数据库的生物信息学分析表明，miR-1225-5p作为一个有利的预后因素，在胶质母细胞瘤中低表达，且其表达还与世界卫生组织（WHO）分级和年龄相关。随后的CCK-8实验表明，miR-1225-5p可能会阻止胶质母细胞瘤的恶性进展，这表现为miR-1225-5p模拟物降低了胶质母细胞瘤细胞的活力。然后，我们预测[此处原文缺失具体基因名称]可能是miR-1225-5p的一个潜在靶基因，并且它与miR-1225-5p的水平呈负相关，这通过双荧光素酶报告基因检测、qRT-PCR和蛋白质免疫印迹实验得到了证实。此外，基于癌症基因组图谱（TCGA）、Oncomine和CGGA数据库的分析，[此处原文缺失具体基因名称]在胶质母细胞瘤中富集，且[此处原文缺失具体基因名称]的高表达导致预后不良。最后，CCK8和Transwell实验表明，miR-1225-5p抑制胶质母细胞瘤细胞活力、侵袭和迁移的能力至少部分是通过靶向[此处原文缺失具体基因名称]实现的。总体而言，这些结果揭示了miR-1225-5p/[此处原文缺失具体基因名称]轴有助于抑制胶质母细胞瘤细胞的恶性表型，这为胶质母细胞瘤的分子诊断和治疗奠定了基础。
