Ma Qiang, Cai Ming, Shang Jing-Wei, Yang Jun, Gu Xin-Yi, Liu Wen-Bo, Yang Qing
School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, Dalian 116023, Liaoning Province, China.
Department of Neurology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China.
Open Med (Wars). 2020 Sep 30;15(1):954-961. doi: 10.1515/med-2020-0229. eCollection 2020.
Bone marrow stromal cells (BMSCs) have an important application prospect in the field of cell therapy for various neurodegenerative diseases, and inducing factors that regulate BMSC differentiation are proposed as a promising therapeutic strategy. In this study, we explored the effect of glial cell-derived neurotrophic factor (GDNF) on the course of BMSC differentiation.
BMSCs were isolated from rat bone marrow and induced by GDNF. The effects of GDNF on BMSC viability and proliferation were verified by cell counting kit-8, MTT, bromodeoxyuridine, and flow cytometry assays. Neuronal differentiation from BMSCs was detected by quantitative real-time polymerase chain reaction and immunofluorescence via measuring the expression of several neural specific markers.
Compared to untreated BMSCs, GDNF induced the differentiation of BMSCs into neuron-like cells and enhanced the expression levels of neuronal markers including nestin and NCAM. Moreover, the expression of SCF was suppressed by GDNF stimulation.
GDNF could elevate the differentiation of BMSCs into neuron-like cells and could be considered as an effective candidate cell for future neuroscience research.
骨髓基质细胞（BMSCs）在各种神经退行性疾病的细胞治疗领域具有重要的应用前景，调控BMSC分化的诱导因子被认为是一种有前景的治疗策略。在本研究中，我们探讨了胶质细胞源性神经营养因子（GDNF）对BMSC分化过程的影响。
从大鼠骨髓中分离BMSCs，并由GDNF诱导。通过细胞计数试剂盒-8、MTT、溴脱氧尿苷和流式细胞术检测GDNF对BMSC活力和增殖的影响。通过定量实时聚合酶链反应和免疫荧光法，通过测量几种神经特异性标志物的表达，检测BMSCs向神经元的分化。
与未处理的BMSCs相比，GDNF诱导BMSCs分化为神经元样细胞，并提高了包括巢蛋白和神经细胞黏附分子在内的神经元标志物的表达水平。此外，GDNF刺激抑制了SCF的表达。
GDNF可提高BMSCs向神经元样细胞的分化，可被视为未来神经科学研究的有效候选细胞。
