Balazs Evelin, Galik-Olah Zita, Galik Bence, Bozso Zsolt, Kalman Janos, Datki Zsolt
Department of Psychiatry, Faculty of Medicine, University of Szeged, Szeged, Hungary.
Bioinformatics Research Group, Bioinformatics and Sequencing Core Facility, Szentágothai Research Centre, University of Pécs, Pécs, Hungary.
IBRO Rep. 2020 Oct 6;9:319-323. doi: 10.1016/j.ibror.2020.10.002. eCollection 2020 Dec.
Investigation of human neurodegeneration-related aggregates of beta-amyloid 1-42 (Aβ42) on bdelloid rotifers is a novel interdisciplinary approach in life sciences. We reapplied an organ size-based monitoring system, exploring the autocatabolism-related alterations evoked by Aβ42, in a glucose-supplemented starvation model. The experientially easy-to-follow size reduction of the bilateral reproductive organ (germovitellaria) in fasted rotifers was rescued by Aβ42, serving as a nutrient source- and peptide sequence-specific attenuator of the organ shrinkage phase and enhancer of the regenerative one including egg reproduction. Recovery of the germovitellaria was significant in comparison with the greatly shrunken form. In contrast to the well-known neurotoxic Aβ42 (except the bdelloids) with specific regulatory roles, the artificially designed scrambled version (random order of amino acids) was inefficient in autocatabolism attenuation, behaving as negative control. This native Aβ42-related modulation of the 'functionally reversible organ shrinkage' can be a potential experiential and supramolecular marker of autocatabolism .
研究β-淀粉样蛋白1-42（Aβ42）在蛭形轮虫中与人类神经退行性变相关的聚集体，是生命科学中一种新的跨学科方法。我们重新应用了一种基于器官大小的监测系统，在补充葡萄糖的饥饿模型中，探索由Aβ42引起的与自身分解代谢相关的变化。在禁食的轮虫中，双边生殖器官（胚卵黄腺）在实验中易于观察到的尺寸减小被Aβ42挽救，Aβ42作为一种营养源和肽序列特异性的器官收缩期衰减剂以及包括卵子繁殖在内的再生期增强剂。与严重萎缩的形态相比，胚卵黄腺的恢复是显著的。与具有特定调节作用的众所周知的神经毒性Aβ42（蛭形轮虫除外）相反，人工设计的乱序版本（氨基酸随机排列）在自身分解代谢衰减方面效率低下，作为阴性对照。这种与天然Aβ42相关的“功能可逆器官收缩”调节可能是自身分解代谢的潜在实验和超分子标志物。
