Department of Otolaryngology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Eur Rev Med Pharmacol Sci. 2020 Dec;24(23):12041-12049. doi: 10.26355/eurrev_202012_23993.
We investigated the effect of electrical stimulation (ES) of varying pulse frequency on differentiation and proliferation of canine myloglossus satellite cells in vitro.
Cellular viability and proliferation were assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay and flow cytometry fluorescence-activated cell sorting analysis. Cellular differentiation and expression of mark molecule were assayed by Real Time-PCR and Western blot.
With increasing frequency ES, we found a significant increase in Myod (r=0.988, p<0.0001), myogenin (r=0.988, p<0.0001), MyHC-slow (r=0.988, p<0.0001), MyHC-fast (r=0.875, p<0.0001) protein expression, and Pax7 mRNA expression (r=0.712, p=0.001).
Pax7 mRNA expression and MyoD, myogenin, and MyHC protein expression were increased with increment of electrical stimulation frequency in myloglossus muscle satellite. Higher frequency ES enhanced myloglossus satellite cell differentiation, not proliferation and viability.
我们研究了不同脉冲频率的电刺激（ES）对体外犬肌卫星细胞分化和增殖的影响。
使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法和流式细胞术荧光激活细胞分选分析检测细胞活力和增殖。通过实时 PCR 和 Western blot 检测细胞分化和标记分子的表达。
随着 ES 频率的增加，我们发现 Myod（r=0.988，p<0.0001）、myogenin（r=0.988，p<0.0001）、MyHC-slow（r=0.988，p<0.0001）和 MyHC-fast（r=0.875，p<0.0001）蛋白表达以及 Pax7 mRNA 表达（r=0.712，p=0.001）显著增加。
随着肌卫星肌 ES 频率的增加，Pax7 mRNA 表达以及 MyoD、myogenin 和 MyHC 蛋白表达增加。更高频率的 ES 增强了肌卫星细胞的分化，而不是增殖和活力。
