Department of Hepatic Surgery, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, P.R. China.
Eur Rev Med Pharmacol Sci. 2020 Dec;24(23):12153-12160. doi: 10.26355/eurrev_202012_24004.
This study aimed to observe the effect of microRNA-7 on the proliferation and migration of human hepatocellular carcinoma cell line SMMC-7721 and its effect on epithelial-mesenchymal transition (EMT).
Eukaryotic expression vector pcDNA3.1(-)-pri-miR-7(p-miR-7) was used to instantaneously transfect human liver cancer cells of SMMC-7721 cells in vitro. The expression of microRNA-7 was detected by RT-qPCR. Western blot was used to detect the expression of EMT marker proteins E-cadherin, β-catenin, N-cadherin and Vimentin. The proliferation of SMMC-7721 cells was detected by CCK-8 assay, and the invasion and migration ability of cells was detected by transwell assay.
Compared with the normal group, the expressions of E-cadherin and β-catenin in SMMC-7721 cells transfected with miR-7 were significantly increased (p<0.05), while the expressions of N-cadherin and Vimentin were significantly decreased (p<0.05). Meanwhile, the proliferation, invasion and migration ability of the cells were significantly weakened (p<0.05).
The miR-7 can inhibit the proliferation and invasion of human hepatocellular carcinoma cell line SMMC-7721, and its mechanism may be related to upregulation of E-cadherin, β-catenin protein, and downregulation of N-cadherin and Vimentin proteins.
本研究旨在观察微小 RNA-7 对人肝癌细胞系 SMMC-7721 增殖和迁移的影响及其对上皮间质转化（EMT）的影响。
采用真核表达载体 pcDNA3.1(-)-pri-miR-7(p-miR-7)瞬时转染人肝癌 SMMC-7721 细胞，通过 RT-qPCR 检测 microRNA-7 的表达。Western blot 检测 EMT 标志物蛋白 E-cadherin、β-catenin、N-cadherin 和 Vimentin 的表达。CCK-8 法检测 SMMC-7721 细胞的增殖，Transwell 法检测细胞的侵袭和迁移能力。
与正常组相比，miR-7 转染的 SMMC-7721 细胞中 E-cadherin 和β-catenin 的表达明显增加（p<0.05），而 N-cadherin 和 Vimentin 的表达明显降低（p<0.05）。同时，细胞的增殖、侵袭和迁移能力明显减弱（p<0.05）。
miR-7 可抑制人肝癌细胞系 SMMC-7721 的增殖和侵袭，其机制可能与上调 E-cadherin、β-catenin 蛋白，下调 N-cadherin 和 Vimentin 蛋白有关。
